Transcriptome Analysis of Germinated Tartary Buckwheat Based on High-throughput Sequencing Technology

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.006

Abstract

Abstract: Illumina SolexaHiseq 2500 high-throughput sequencing technology was used to get the comprehensive transcriptome from germinated tartary buckwheat. As a result,42 953 962 sequence reads containing 5.37 Gb nucleotide sequence information were obtained. After de novo assembly by the software of Trinity,a total of 45 278 unigenes were generated,corresponding to a total of 39 Mb with an average length 862 bp. In addition,the data from the evaluation of the unigenes indicated fine sequencing quality and high reliability from the aspects of length distribution,GC content,and expression level. The comparison of sequence homology in database showed that 2 127 unigenes had various degrees of homology with other known biological genes. The unigenes in the transcriptome of germinated tartary buckwheat were correlated with cellular processes,cell and protein binding. According to KOG database,the unigenes were broadly divided into 24 categories. Referring to KEGG database,unigenes were located into 328 metabolic pathways,including ribosome,carbohydrate metabolism and so on. And 38 unigenes involved in the synthesis of GABA in oxidative phosphorylation metabolism were screened. Total 7 141 unigenes were found from 71 366 by SSR and the highest frequency was A/T,followed by AAG/CTT and AT/AT.

Key words: germinated tartary buckwheat, Illumina, transcriptome, high-throughput sequencing technology

CHEN Chun-xu, LI Qi, GUO Yuan-xin, DU Chuan-lai, DING Zhi-gang. Transcriptome Analysis of Germinated Tartary Buckwheat Based on High-throughput Sequencing Technology[J]. Biotechnology Bulletin, 2016, 32(7): 40-47.

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