Application of Single-cell Transcriptome Sequencing in Animals

doi:10.13560/j.cnki.biotech.bull.1985.2021-0523

Abstract

Abstract:

Cell is the basic structural component and functional unit of organism. Cell type and function are determined by its whole transcriptional expression profile. Single-cell transcriptome sequencing can be used to obtain the transcriptional expression profile of a single cell,so as to identify cell types,cell states and rare types with high-precision resolution,thus to analyze the dynamic changes of cells and the relationship between cells at the single cell level,and further to decipher the molecular cellular mechanisms behind cell changes and cell abnormalities. With the stability and throughout improvement of single-cell sequencing,as well as the reduction of sequencing cost,single-cell transcriptome sequencing has been widely used in the fields of developmental biology,tumor,immunity and disease. However,these researches are mainly focused on human and model organisms,there are a few researches on animals. Therefore,the objective of this paper is to introduce the single-cell transcriptome sequencing and its biological application,and review some of its pioneering research in animals,aiming to provide a method reference for better application of single-cell transcriptome sequencing in animals.

Key words: single-cell transcriptome sequencing, cell type, biological application, animals

XIONG He-li, SHA Qian, LIU Shao-na, XIANG De-cai, ZHANG Bin, ZHAO Zhi-yong. Application of Single-cell Transcriptome Sequencing in Animals[J]. Biotechnology Bulletin, 2022, 38(3): 226-233.

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