Isolation and Identification of Streptomyces triostinicus C2 Antagonizing on a Variety of Plant Pathogenic Fungi

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.016

Abstract

Abstract: The research purposes of this work are to isolate and screen a strain against phytopathogenic fungi. The antagonistic strain was isolated and screened using plate confrontation culture with Rhizoctonia solani as indicator,and was further identified on the basis of morphological characteristics,physiological and biochemical characterization,and 16S rDNA sequence analysis. From 18 soil samples collected from Yunnan,Guangdong,Anhui,Hubei,and Jiangxi Provinces of China,the isolated actinomycete strain C2 presented a strong inhibitory effect on R. solani,and it was preliminarily identified as Streptomyces triostinicus,designated as Streptomyces strain C2 here. The bioassay results showed that Streptomyces strain C2 possessed a broad-spectrum inhibitory effect on a range of plant fungi such as R. solani,Pyricularia grisea,Colletotrichum gloeosporioides,Fusarium oxysporum f. sp. niveum,and Penicillium citrinum,and the inhibition percentage was 49.78%,56.62%,80.96%,18.59%,and 94.10%,respectively. Conclusively,Streptomyces strain C2 was an antagonistic strain with broad-spectrum in suppressing the growth of various plant fungal pathogens.

Key words: isolation and identification, actinomycete strain C2, phytopathogenic fungi, broad-spectrum antagonist

PENG Wei-fu, WU Zhi-ming, CHEN Wei, ZENG Yong-jun, LI Kun-tai. Isolation and Identification of Streptomyces triostinicus C2 Antagonizing on a Variety of Plant Pathogenic Fungi[J]. Biotechnology Bulletin, 2016, 32(7): 106-111.

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