Abstract: Mycoparasite Pestalotiopsis produces the second metabolites different from endophytic or pathogenic Pestalotiopsis,as an important fungal resource,is yet to be developed and used. In order to obtain highly active mycoparasites ofAecidium pourthiaea,tissue separation method was used to isolate the mycoparasites,and 3 strains were isolated from the leaves of Photinia prionophylla with rust and suspected to be Pestalotiopsis;moreover,they were confirmed to be mycoparasites of A. pourthiaea via the tie back experiments though their morphologies varied obviously. Cultural characteristics on different medium and microscopic features of these three strains were described;also ITS sequences were amplified and analyzed. The morphology and molecular identification confirmed that all 3 strains were Pestalotiopsis. The results of anti-fungus experiment showed that these three strains not only destroyed the aecidiospores of A. pourthiaea,but also inhibited the growth of other 10 plant pathogenic fungi.

Key words: Pestalotiopsis, mycoparasite, isolation and identification, antimicrobial activity, Aecidium pourthiaea