Correlations Between Microbial Community and Physicochemical Indexes in Pit Mud of Different Ages

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.018

Abstract

Abstract: This experiment aims to study the diversity of major microbial community structure in pit mud of different ages and the variation pattern of pit mud’s physical and chemical index（pH,total nitrogen,humic acid,available phosphorus and total acid）,and to analyze the correlations among them. The results of Kjeldahl determination showed that：with the increase of pit age,pH of pit mud did not change basically,total acid tended to decrease slowly,total nitrogen rose firstly and then dropped,humic acid and available phosphorus increased slowly. The diversity index of bacteria and archaea by DGGE ranged as 1.62-2.58 and 1.79-2.33,respectively,and gradually increased with the increasing of pit age. The correlation coefficient between bacteria’s community structure and humic acid was 0.972（P<0.01）,-0.987（P<0.01）with total acid,and 0.878（P<0.05）with available phosphorus. The correlation coefficient of archaea’s community structure and humic acid was 0.986（P<0.01）,-0.954（P<0.05）with total acid,0.901（P<0.05）with available phosphorus,and no significant correlation with other indexes. Regarding bacteria,selected 11 dominant bands for sequencing,and most of them were found to be bacillus generally. Regarding archaea,selected 4 dominant bands for sequencing,and most of them were found to be methanogen. The physiochemical indexes and microbial community structure in pit mud showed a certain regular pattern with the change of the pit age,and some physiochemical indexes were strongly correlated with microbial community structure,these physiochemical indexes greatly concerned with the microbe’s domestication.

Key words: pit mud, PCR-DGGE, microbial community, correlation analysis

ZHONG Shu-xia DENG Jie WEI Chun-hui LUO Hui-bo WAN Shi-lü HUANG Zhi-guo. Correlations Between Microbial Community and Physicochemical Indexes in Pit Mud of Different Ages[J]. Biotechnology Bulletin, 2016, 32(7): 119-125.

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