Effects of Soil Moisture on the Microbial Community Under Continuous Cropping of Panax notoginseng

doi:10.13560/j.cnki.biotech.bull.1985.2021-1287

Abstract

Abstract:

Using 8-year replanting P. notoginseng soil as study object,we investigated the effects of SMC（10%,20%,30% and 40%）on the soil physicochemical properties and microbial community by using the high-throughput DNA sequencing and in-door soil-cultivated experiment. The results showed that the relative abundance of Fusarium was the highest when the SMC was maintained at 10% and 20%. However,when SMC was 30%,the relative abundance of Fusarium decreased and that of Trichoderma increased significantly（P<0.05）,which was benefit to controlling the soil-borne diseases of P. notoginseng. When SMC was raised to 40%,the relative abundance of Fusarium increased while Trichoderma decreased（P<0.05）. Comparing to bacterial community,the fungal community varied more obviously with the increase of SMC. Moreover,when SMC was maintained at 40%,the content of NH₄⁺-N significantly increased,while NO₃^--N content decreased（P<0.05）. Our study provided scientific basis for reducing the obstacle of replanting P. notoginseng from the perspective of field moisture management.

Key words: Panax notoginseng, soil moisture content, replanting obstacle, physicochemical properties, microbial community

ZHAO Lin-yan, GUAN Hui-lin, WANG Ke-shu, LU Yan-lei, XIANG Ping, WEI Fu-gang, YANG Shao-zhou, XU Wu-mei. Effects of Soil Moisture on the Microbial Community Under Continuous Cropping of Panax notoginseng[J]. Biotechnology Bulletin, 2022, 38(7): 215-223.

Figures/Tables 9

Fig. 1 Relative abundances of microorganisms at the phyl-um level under different soil moisture treatments A：Fungal community;B：bacterial community,W1,W2,W3,and W4 indicate the soil moisture content at 10%,20%,30%,and 40% respectively,the same below

Fig. 2 Relative abundances of soil representative microbial genera under different soil moisture treatments Different letters indicate significant difference at P<0.05

Fig. 3 Number of shared and unique OTUs under different soil moisture treatments A：Fungal community;B：bacterial community

Fig. 4 Rarefaction curves for fungal and bacterial OTU richness under different soil moisture treatments A：Fungal community;B：bacterial community

Fig. 5 Alpha diversities of soil microbial community under different soil moisture treatments

Fig. 6 NMDS and ANOSIM results of microbial communities under different treatments based on Bray-Curtis distance A and C：The results of NMDS and ANOSIM of fungal community;B and D：the results of NMDS and ANOSIM of bacterial community

Table 1 Results of one-way ANOVA for the soil physico-chemical properties under continuous cropping of P. notoginseng with different moisture treatments

土壤理化性质 Soil property	SS	df	s²	F	P
pH	0.03	3	0.01	1.76	0.23
EC	22 856.33	3	7 618.78	1.53	0.28
NH₄⁺-N	11.42	3	3.81	17.30	<0.01
NO₃^--N	1 099.20	3	366.40	32.27	<0.01
AP	0.31	3	0.10	0.86	0.50
AK	789.12	3	263.04	1.09	0.41

Fig. 7 Effects of different soil moisture treatments on the contents of soil NH4+-N and NO3--N

Fig. 8 RDA analysis of soil fungal（A）and bacterial com-munity（B）under different moisture treatments

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