The Role of Myrosinase TGG1 in Drought Resistance in  Arabidosis thaliana

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.029

Abstract

Abstract: In order to illuminate the function of myrosinase TGG1 in drought resistance,an over-expressing vector with TGG1 gene driven by 35S promoter was constructed and transferred into Arabidopsis thaliana. Drought stress were tested using the wild and TGG1-overexpressed transgenic plants. Our results showed that in a simulated drought stress by the treatment of mannitol,the seed germination rate of transgenic seedlings was significantly higher than those of wild type,while relative conductivity rate of leaves in transgenic plants was significantly lower than that in wild type. In the condition of natural drought stress followed by re-watering,35S∶TGG1 showed higher survival rate and was more sensitive to ABA in the process of stomatal closure,and consequently presented the decreased water loss rate and permeability of cell membrane. In general,our study suggested that overexpressed TGG1 gene improved the drought tolerance of A. thaliana,and the mechanism is likely related to degree of stomata closing in stress.

Key words: myrosinase, TGG1, Arabidopsis thaliana, drought stress, stomata

MAO Ming-yu¹, ZHANG Wen-yu¹, XIA Hong-yu¹, WANG Yang², LI Jing¹. The Role of Myrosinase TGG1 in Drought Resistance in Arabidosis thaliana[J]. Biotechnology Bulletin, 2016, 32(9): 218-224.

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The Role of Myrosinase TGG1 in Drought Resistance in Arabidosis thaliana

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