Identification of FtCBL Genes in Fagopyrum tataricum and Their Stress Responses to Drought and High Calcium

doi:10.13560/j.cnki.biotech.bull.1985.2021-1367

Abstract

Abstract:

Plant CBL genes play important roles in stress response. It is of great significance to identify FtCBL genes of tartary buckwheat（Fagopyrum tataricum L.）and to explore its responses to drought and high calcium stress. Bioinformatics methods were conducted to identify the FtCBL gene of tartary buckwheat. The expression of FtCBL gene under drought,high calcium and combined drought and high calcium treatment was investigated by mimicing stress. A total of 5 FtCBL genes with conserved EF-hand calcium-binding domain were identified,which were located at chromosome Ft3（2）,Ft4（1）and Ft5（2）. Their cDNA length was 642-684 bp,encoding 213-227 amino acids with highly conserved sequence. The CAT and APX enzyme activities of tartary buckwheat increased sharply under drought,but the difference was insignificant under high calcium,indicating that tartary buckwheat was relatively suitable for growing under high calcium environment. Under drought stress,FtCBL1 was down-regulated at 8 and 24 h,but FtCBL2 at 8 and 24 h and FtCBL3-1 at 8 h were up-regulated. Under high calcium stress,FtCBL1 at 8 and 24 h and FtCBL9 at 24 h were down-regulated,whereas FtCBL2 was up-regulated. Under combined drought and high calcium stress,FtCBL3-1 was up-regulated at 8 and 24 h. A total of 5 FtCBL genes are identified in tartary buckwheat. FtCBL1 and FtCBL2 are induced by drought stress and high calcium stress,respectively,FtCBL3-1 is induced by drought stress and combined drought and high calcium stress,and FtCBL9 is induced by high calcium stress.

Key words: Fagopyrum tataricum, calcineurin B-like protein, drought stress, high-calcium stress, drought and high-calcium stress

GUAN Zhi-xiu, WANG Yan, LIANG Cheng-gang, WEI Chun-yu, HUANG Juan, CHEN Qing-fu. Identification of FtCBL Genes in Fagopyrum tataricum and Their Stress Responses to Drought and High Calcium[J]. Biotechnology Bulletin, 2022, 38(8): 101-109.

Figures/Tables 9

References 42

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基因编号Gene No.	引物序列 Primer sequence（5'-3'）
FtPinG0000638500.01	F	CTGTGTCTCTTGCCTCCC	R	CAATGGCTCCCTTGTGCT
FtPinG0003042400.01	F	ACTTTCTGTGTTCCATCC	R	TACCACCATTTGCTTTAC
FtPinG0003981900.01	F	GGCACGAGGTCAAGCAAATG	R	TTGTCGCCATTCGGATCTGT
FtPinG0007340500.01	F	AGCATTTCCTCGCTTCGT	R	CATCGTCAATCACCGCAC
FtPinG0008915000.01	F	CTTTCTGTTTTCCATCCG	R	TCGTCCTTGTCAATCCTT
FtActin	F	gaaattcgcaagtaccagaagag	R	ccaacaaggtatgcctcagc

基因编号Gene No.	引物序列 Primer sequence（5'-3'）
FtPinG0000638500.01	F	CTGTGTCTCTTGCCTCCC	R	CAATGGCTCCCTTGTGCT
FtPinG0003042400.01	F	ACTTTCTGTGTTCCATCC	R	TACCACCATTTGCTTTAC
FtPinG0003981900.01	F	GGCACGAGGTCAAGCAAATG	R	TTGTCGCCATTCGGATCTGT
FtPinG0007340500.01	F	AGCATTTCCTCGCTTCGT	R	CATCGTCAATCACCGCAC
FtPinG0008915000.01	F	CTTTCTGTTTTCCATCCG	R	TCGTCCTTGTCAATCCTT
FtActin	F	gaaattcgcaagtaccagaagag	R	ccaacaaggtatgcctcagc

基因编号 Gene No.	序列长度Sequence length/bp	氨基酸Amino acid/aa	分子量Molecular weight/Da	等电点pI	脂肪族氨基酸指数 Aliphatic index	染色体 Chromosome	α-螺旋 Alpha helix/%	延长链 Extended strand/%	β-折叠 Beta turn/%	无规卷曲 Random coil/%
FtPinG0003981900.01	642	213	24 343.7	4.8	86.01	Ft3	49.30	7.51	7.51	35.68
FtPinG0007340500.01	684	227	26 265.9	4.9	90.62	Ft3	54.63	6.17	8.37	30.84
FtPinG0003042400.01	678	225	25 755.3	4.8	89.69	Ft5	56.00	4.44	7.11	32.44
FtPinG0008915000.01	681	226	25 887.4	4.8	90.13	Ft4	53.98	7.08	7.08	31.86
FtPinG0000638500.01	642	213	24 305.5	4.7	86.95	Ft5	51.17	6.57	6.10	36.15

基因编号 Gene No.	序列长度Sequence length/bp	氨基酸Amino acid/aa	分子量Molecular weight/Da	等电点pI	脂肪族氨基酸指数 Aliphatic index	染色体 Chromosome	α-螺旋 Alpha helix/%	延长链 Extended strand/%	β-折叠 Beta turn/%	无规卷曲 Random coil/%
FtPinG0003981900.01	642	213	24 343.7	4.8	86.01	Ft3	49.30	7.51	7.51	35.68
FtPinG0007340500.01	684	227	26 265.9	4.9	90.62	Ft3	54.63	6.17	8.37	30.84
FtPinG0003042400.01	678	225	25 755.3	4.8	89.69	Ft5	56.00	4.44	7.11	32.44
FtPinG0008915000.01	681	226	25 887.4	4.8	90.13	Ft4	53.98	7.08	7.08	31.86
FtPinG0000638500.01	642	213	24 305.5	4.7	86.95	Ft5	51.17	6.57	6.10	36.15