Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (12): 96-102.

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Phylogeography of Gymnadenia conopsea from the Qinghai-Tibet Plateau

BAO Wu-yin1,ZHANG Yang2,LIN Peng-cheng3,NAN Peng1,HUANG Yan-yan1,JIN Hao-fei1,ZHONG Yang1   

  1. 1. Molecular and Evolutionary Ecology Lab,School of Life Science,Fudan University,Shanghai 200438;
    2. Natural History Research Center,Shanghai Science and Technology Museum,Shanghai 200041;
    3. Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province,Qinghai University for Nationalities,Xining 810007
  • Received:2016-06-03 Online:2016-12-25 Published:2016-12-07

Abstract: Chloroplast DNA(rbcl and psbA-trnH)of Gymnadenia conopsea(G. conopsea)from the Qinghai-Tibet Plateau was used for phylogeography research,aiming at revealing the distribution pattern and evolutional process of genetic lineage of G. conopsea. Eleven haplotypes were identified in 199 samples of 10 G. conopsea populations. The analysis of molecular variance(AMOVA)showed 65.93% of the genetic variance was from inter-populations and FST value of 0.659(P<0.01)also indicated that there was significant genetic differentiation between different populations. The genetic differentiation coefficients NST(0.398)< GST(0.626)implied no significant phylogeographic structure for G. conopsea populations in the Qinghai-Tibet Plateau. Mismatch analysis of multi-peak and Tajima’s D neutral test(-1.455,P>0.1)demonstrated G. conopsea populations had not experienced abrupt expansion recently. Phylogenetic analysis and divergence time estimation showed that G. conopsea in the Qinghai-Tibet Plateau originated indigenously,haplotypes started to differentiate 19.08 million years ago(Mya)in Miocene,indicating that the genetic differentiation of G. conopsea was related to the uplift of the Qinghai-Tibetan Plateau,and the genetic distribution pattern of G. conopsea was mostly formed before the Quaternary glaciation.

Key words: Gymnadenia conopsea, phylogeography, genetic diversity, the Qinghai-Tibet Plateau

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