The Expression of Transcription Factor GATA4 Gene in Takifugu rubripes

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.022

Abstract

Abstract: In this study，GATA4 expression were analyzed in kidney，muscle，brain，spleen，liver，testis and ovary of mature Takifugu rubripes，and were analyzed at individual level during different times in young T. rubripes by flurescence quantitative Real-time Polymerase Chain Reaction(qRT-PCR). The results showed that no statistically significant difference was observed of expression level among muscle，spleen，kidney and brain. There was the significantly higher expressionof GATA4 in testis ，liver and ovary，than that in other organs(P<0.05). The GATA4 expression level of testis and liver were significantly higher(P<0.05)，than that in ovary. During the different periods(23，30，40，60，80 days of age(d)) of T. rubripes，the GATA4 expression level in males increased gradually from 23 d to 30 d and subsequently decreased at 40 d，and the 30 d expression level of GATA4 was higher than that in 30 d female individuals significantly(P<0.01). While from 60 d to 80 d，the GATA4 expression level went steadily up. In females，as the same as the expression tend in males，the GATA4 expression level increased gradually from 23 d to 40 d and subsequently decreased at 60 d，and the 40 d GATA4 gene expressed higher than that in the same time male individuals significantly(P<0.01). Nevertheless from 80 d，the GATA4 expression level stared to rise. Apart from 40 d individuals，the GATA4 expression level were higher in males than in female in every period.

Key words: Takifugu rubripes, quantitative Real-time PCR, transcription factor GATA4, gene expression

HAO Wei-wei, GAO Chang-fu, QIU Xue-mei, JIANG Zhi-qiang, WANG Xiu-li. The Expression of Transcription Factor GATA4 Gene in Takifugu rubripes[J]. Biotechnology Bulletin, 2016, 32(6): 155-160.

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