Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (3): 175-179.doi: 10.13560/j.cnki.biotech.bull.1985.2017.03.025

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miR-124 Regulates Porcine Adipocyte Differentiation Trough Targeting TNF-α

LI Hong-yi1, LI Jia-biao1, ZHENG Yi-lin1, JI Lu-lin1, ZHANG Mao1, ZHENG En-qin2   

  1. 1.Animal Nutrition Scientific Research Innovation Team,College of Life Science,Longyan University,Longyan 364012;
    2.Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642
  • Received:2016-08-02 Online:2017-03-26 Published:2017-03-07

Abstract: In the previous research,porcine TNF-α was found to be the target of miR-124 by dual luciferase assay. Here we verify whether miR-124 has the effect on the differentiation of porcine adipocyte. Porcine pre-adipocyte was transfected with miR-124 mimics or inhibitor to over-express or to repress miR-124,and then induced into mature adipocytes. The accumulation of lipid droplets was observed using Oil Red O staining and the amount of glycerol and triglycerides(TG)were detected by TG assay and glycerol assay. The expressions of PPARγ(proliferator-activated receptor-γ),FASN(fatty acid synthase),and HSL(hormone-sensitive lipase)were detected by real time fluorescence quantitative PCR. The result showed that overexpression of miR-124 repressed the expression of TNF-α protein,therefore,lipid droplets were more than that in the control,TG increased significantly(P < 0.01),also the same for glycerol(P < 0.05),and the expressions of PPARγ,FASNT,and HSL significantly up-regulated(P < 0.01). Repressing miR-124 resulted in the reduction of lipid droplets and the significant decrease of TG,and the expressions of PPARγ and FASNT significantly down-regulated(P < 0.05). In conclusion,miR-124 might regulate the differentiation of porcine adipocyte by suppressing TNF-α,laying a foundation for future studies on the mechanism of miR-124 regulating lipid metabolism.

Key words: miR-124, porcine, adipocyte differentiation, TNF-α