Study on Antioxidant and Antitumor Activity of Essential Oil from Flowers of Syringa oblata

doi:10.13560/j.cnki.biotech.bull.1985.2019-0144

Abstract

Abstract: Essential oil in the flowers of Syringa oblata at the bud stage was extracted by hydro distillation,and 3 different antioxidant tests were employed to evaluate the antioxidant activities of the essential oil. The scavenging effects of the essential oil at different concentrations on DPPH radical and hydroxyl free radicals were measured,and the total antioxidant capacity（T-AOC）of the essential oil was also measured. The results indicated that the essential oil had a favorable scavenging effect on DPPH free radical and hydroxyl radical in a dose-dependent manner. The T-AOC of the essential oil was 19.86±1.74 U/mg,higher than that of 3,5-Di-tert-butyl-4-hydroxytoluene（0.38±0.08 U/mg）. Gastric cancer lines MGC80-3 and HGC-27 were used to access the inhibitory effect of the essential oil by MTT（3-（4,5-Dimethyl-2-thiazolyl）-2,5-diphenyl-2H- tetrazolium bromide）method. The apoptotic index of the essential oil was detected by TUNEL assay. The results of MTT assay indicated that the essential oil significantly inhibited MGC80-3 and HGC-27 in a dose-dependent manner,and the IC₅₀ values,after 48 h exposure,were 0.282 mg/mL and 0.263 mg/mL respectively. TUNEL assay indicated the essential oil induced apoptosis also in a dose-dependent manner,and HGC-27 cells showed more sensitive than MGC80-3 cells. Therefore,essential oil in the flowers of S. oblata shows remarkably high antioxidative and antitumor activities in vitro,and demonstrated potential value of medical application.

Key words: Syringa oblata flowers, essential oil, antioxidant, anti-tumor, apoptosis

HU Jian-ran, LI Ping, TIE Jun, JIN Shan. Study on Antioxidant and Antitumor Activity of Essential Oil from Flowers of Syringa oblata[J]. Biotechnology Bulletin, 2019, 35(12): 16-23.

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