Identification of Candidate Genes Related to the Development of Pleurotus tuoliensis Fruiting Bodies

doi:10.13560/j.cnki.biotech.bull.1985.2018-0139

Abstract

Abstract: Pleurotus tuoliensis contains rich nutrition and has many functions such as anti-tumor and enhancing immunity,etc.,thus it is precious edible white mushroom with independent intellectual property right in China. Identifying the candidate genes related to the formation of P. tuoliensis fruiting bodies would provide effective and rapid markers for its breeding. Two strains were obtained from the previous studies,one strain JZB2106010 formed fruiting bodies,while another dikaryotic hybrid strain JZB2106103 from protoplast monokaryons of JZB2106010 couldn’t form fruiting body. The transcriptome sequencing of JZB2106010 and JZB2106103 were performed to identify the differentially-expressed genes and functional enrichment analysis was carried out. GO enrichment displayed that there were the most differentially-expressed genes in catalytic activity and metabolic process. qPCR verification of partially the differentially-expressed genes showed that phenylalanine ammonium lyase（CL4509）and aryl-alcohol oxidase gene（CL2173）had significant high gene expressions in JZB2106010,revealing that CL4509 and CL2173 were related to the development of P. tuoliensis fruiting bodies. In addition,ATP dependent DNA helicase（Unigene7793）displayed 1 046-fold higher expression in JZB2106103 than in JZB2106010,inferring that Unigene7793 was negatively related to the development of P. tuoliensis fruiting bodies.

Key words: Pleurotus tuoliensis, development of fruiting bodies, transcriptome, phenylalanine ammonium lyase, aryl-alcohol oxidase, DNA helicase

RONG Cheng-bo, ZHAO Shuang, SONG Shuang, WANG Jing, LIU Yu. Identification of Candidate Genes Related to the Development of Pleurotus tuoliensis Fruiting Bodies[J]. Biotechnology Bulletin, 2018, 34(4): 115-120.

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