Isolation and Degradation Characteristics of a Efficient Tetracycline-degrading Strain

doi:10.13560/j.cnki.biotech.bull.1985.2017-0723

Abstract

Abstract: The wide use of tetracycline antibiotics in aquaculture causes drug residues,which brings serious impacts to environment and human health. In this study,a strain XY-1,which efficiently degraded tetracycline,was screened from the sediment of pig farm using tetracycline antibiotics as feed additive. After the Gram stain,morphological observation,physiological and biochemical and 16S rDNA sequence analysis,the strain was identified as Raoultella sp. and named as XY-1.The ability of strain degrading tetracycline was determined by high performance liquid chromatography（HPLC）. The results showed that the optimal degradation conditions were as followings：the temperature was 25℃ and the initial pH was 7.0. Under the optimal conditions,XY-1 grew at most and the highest tetracycline degradation rate reached 70.68% at the 8th day. Besides,the preliminary probe on the tetracycline-resistant genes was also conducted,and it was the first time to have two tetracycline-resistant genes tet（D）and tet（E）detected in the strain.

Key words: tetracycline-degrading strain, screening and identification, degradation characteristics, tetracycline-resistance gene

WU Xue-ling, WU Xiao-yan, LI Jiao-kun, SHEN Li, YU Run-lan, ZENG Wei-min. Isolation and Degradation Characteristics of a Efficient Tetracycline-degrading Strain[J]. Biotechnology Bulletin, 2018, 34(5): 172-178.

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