Screening of High-yield Rhamnolipid Producing Strain by ARTP and the Effect of Rhamnolipid on the Cellulase and Xylanase

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.025

Abstract

Abstract: In order to obtain an industrial strain with higher rhamnolipid production, the original strain of Pseudomonas aeruginosa C3 was mutated by atmospheric and room temperature plasmas. A good genetic stability strain SC-11 was screened from the mutants, which produced rhamnolipid 74.1% higher than that by the original strain. Through single factor experiments, the culture medium for rhamnolipid production was opttimized. The yield of rhamnolipid could reach 42 g/L and the substrate conversion rate is 0.7 g/g substrates. 0.01% rhamnolipid could enhance CMCase by 12.9% and xylanase by 18.3%. The results show that rhamnolipid increased the production of cellulase by increasing cell permeability.

Key words: atmospheric and room temperature plasmas(ARTP), rhamnolipid, CMCase, xylanase

Chen Lijuan, Wu Bin, He Bingfang. Screening of High-yield Rhamnolipid Producing Strain by ARTP and the Effect of Rhamnolipid on the Cellulase and Xylanase[J]. Biotechnology Bulletin, 2015, 31(11): 195-201.

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