Abstract: Pesticide pollution becomes major cause to environment safety and human health. Introducing microorganisms of degrading specific pesticide into polluted soil may efficiently reduce the concentration of this pesticide;moreover,this is a green technology and causes no secondary pollution. In order to reduce the residual carbendazim in the soil of a greenhouse,a strain WNP-3 decomposing and utilizing carbendazim was isolated via sole carbon source plate screening method from the greenhouse soil where carbendazim has been used for a long time. This strain normally grew in LB medium with carbendazim concentration of 200 mg/L. According to the results of morphology,physiological and biochemical property,Biolog identification,and 16S rDNA sequence analysis,WNP-3 was identified as Ochrobactrum tritici. The WNP-3 had broad growth range,i.e.,growing well at pH 4 - 9 in culture media containing 1%-3% NaCl,and it was resistant to several pesticides. Confirmed by UV spectrophotometry and HPLC,the carbendazim at concentration of 100 mg/L degraded 61% in mineral medium in 180 r/min flask for 120 h at 28℃.

Key words: bacteria, carbendazim, Ochrobactrum tritici, biodegradation