SMRT Sequencing and Its Application in Microorganism Studies

doi:10.13560/j.cnki.biotech.bull.1985.2017-1009

Abstract

Abstract: The development of high-throughput sequencing has provided power for researchers to further explore the microorganism. Along with rapid advances of third generation sequencing（TGS）technologies，especially single molecule real time sequencing（SMRT）developed by Pacific BioSciences（PacBio），methods of studying micro-organism are going through a revolutionary change. SMRT sequencing technology，for its special SMRTbell sequencing libraries and super-long reads length，provides a new choice for sequencing full length of 16S rRNA，and also provides a novel method for acquiring accurate and reliable metagenome and whole genome sequence of microbial species. With the cost of sequencing using SMRT sequencing technology remarkably reduced，a growing number of microbial studies will be performed with the PacBio series platform based on SMRT technology，including sequencing of 16S rRNA gene，metagenome sequencing，and whole genome sequencing. Here，we reviewed the principle and characteristics of SMRT sequencing technology and its application in full length sequencing of 16S RNA，metagenome sequencing，and transcriptome sequencing of microorganism. Further we analyzed the advantages and issues while SMRT sequencing technology is used in studying microorganism，as well as the potential issues of download analysis of SMRT reads. This review is aimed to provide advice or reference for researchers who will employ SMRT sequencing technology into microbial studies.

Key words: third-generation sequencing, SMRT, microorganism

TANG Yong ,LIU Xu. SMRT Sequencing and Its Application in Microorganism Studies[J]. Biotechnology Bulletin, 2018, 34(6): 48-53.

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