Wdr1 Knockout Inhibits Migration and Proliferation of Mouse Primary Vascular Smooth Muscle Cells

doi:10.13560/j.cnki.biotech.bull.1985.2018-0578

Abstract

Abstract: Cytoskeletal actin plays an important role in cell proliferation,migration,and other biological processes. However,the role of WDR1,a major cofactor for actin depolymerizing,has not been reported in smooth muscle cells（SMCs）. Inducible Wdr1 conditional knockout mouse model was constructed,and then sacrificed to isolate mouse primary vascular smooth muscle cells（VSMCs）and induced to detect the morphology,proliferation,and migration. The PCR results suggested that Wdr1^f/f;ERT2Cre mouse model was successfully constructed. The results of immunofluorescence revealed that the improved separation method can effectively separate primary smooth muscle cells. A significant effect on cell morphology after Wdr1 knockout was observed. Concurrently,the scratch and CCK8 test results also showed that Wdr1 conditional knockout obviously inhibited the migration and proliferation of SMCs. The changes of smooth muscle cell morphology and cell biological processes after Wdr1 knockout indicate that it is closely related to the occurrence and development of vascular diseases. It is of great significance to uncover the pathophysiological process of blood vessels.

Key words: conditional induced knockout mouse, WDR1, smooth muscle cells, proliferation, migration

YUAN Bai-yin, HU Ji-sheng, LIU Zhong-ying, HUANG Xia. Wdr1 Knockout Inhibits Migration and Proliferation of Mouse Primary Vascular Smooth Muscle Cells[J]. Biotechnology Bulletin, 2018, 34(12): 179-185.

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