Characterization of the Salt Tolerance of Transgenic Maize Line Expressing ABP9

doi:10.13560/j.cnki.biotech.bull.1985.2019-0007

Abstract

Abstract: This work is to analyze the salt tolerance of ABP9 transgenic maize,and to study the molecular regulation mechanism of salt tolerance in maize. ABP9 transgenic plants were identified by PCR,Southern blot and quantitative Reverse Transcription-PCR（qRT-PCR）,and their responses at seedling stage to NaCl treatments in Hoagland nutrient solution were molecularly and physiologically characterized with non-transgenic plants as control. The results showed that the ABP9transgene was integrated into the genome in a single copy and expressed at a higher level. Compared with the non-transgenic control,chlorophyll content,Fv/Fm,osmotic adjustment substance,and antioxidant enzyme activities in transgenic maize seedlings under NaCl stress,and the relative water content of leaves during recovery after salt stress all significantly increased,while the aldehyde content and relative conductivity significantly reduced. Several salt stress responsive genes were up-regulated in transgenic plants revealed by transcriptome profiling and qRT-PCR analysis. Our results indicate that enhanced ABP9 expression level contributes to salt tolerance in transgenic maize,providing a valuable germplasm for maize salt tolerance breeding.

Key words: maize, ABP9, salt tolerance, transcriptome sequencing

ZHANG Zhao-yang, PANG Jun-ling, HAN Mei, LENG Peng-fei, ZHAO Jun. Characterization of the Salt Tolerance of Transgenic Maize Line Expressing ABP9[J]. Biotechnology Bulletin, 2019, 35(5): 48-57.

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