Influence of mglB Gene Knockout in Engineered Escherichia coli Producing Ethanol on the Xylose Utilization of Mix Sugar Fermentation

doi:10.13560/j.cnki.biotech.bull.1985.2018-0962

Abstract

Abstract: To investigate the influence of glucose non-PTS transferring gene and its synergistic effect with PTS transferring gene on xylose utilization,a glucose transferring gene mglB was knocked out from engineered Escherichia coli SZ470 and its ptsG mutant SZ470P via RED homologous recombination technique,thus mglB-deleted SZ470M and ptsG/mglB-deleted SZ470P were constructed. The fermentations of mix sugar（3% glucose +2% xylose）by 4 strains were compared,and the transcriptional changes of genes related to xylose transferring and metabolism through all 4 strains were also investigated. The results showed that there were no obvious differences between SZ470M and SZ470 in fermentation. The xylose consumption rate,yield and conversion rate of ethanol by SZ470PM were 0.37 g/L,23.25 g/L and 82.6%,respectively,which increased by 32%,9.8% and 5.8% than those in starting strain SZ470P,respectively. The transcriptional analysis also demonstrated that the transcriptional levels of genes related to xylose transferring and metabolism rose in both SZ470M and SZ470PM rose. In sum,the knockout of both ptsG and mglB synergistically increase the xylose utilization rate,which contributes to the efficient utilization of xylose when using lignocelluloses materials for fermentation.

Key words: ethanol fermentation, mglB gene, mix sugar fermentation, xylose utilization

XU Qiong-dan, WANG Yong-ze, WANG Jin-hua, ZHAO Xiao. Influence of mglB Gene Knockout in Engineered Escherichia coli Producing Ethanol on the Xylose Utilization of Mix Sugar Fermentation[J]. Biotechnology Bulletin, 2019, 35(6): 83-90.

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