Research on the Localization and Function of Atp4 in Schizosaccharomyces pombe

doi:10.13560/j.cnki.biotech.bull.1985.2019-0111

Abstract

Abstract: In this study,we studied the localization of Atp4 in Saccharomyces pombe and the mechanism of its involving in the mitochondrial function. The technologies utilized in the article included gene knock,fluorescence microscope,mitochondrial extraction,biochemical treatment,Western blotting,and so on. The Δatp4 deletion mutant glycerin medium grew defectively,and it was mitochondrial respiratory-deficient strain. The Atp4 with GFP label in C terminal showed co-localization with mitochondrion by fluorescence microscope observation. Treating Atp4-Flag and vyHL6381 mitochondria by biochemical reagents TritonX-100 and protease K and sodium carbonate revealed that Atp4 located in mitochondrial endometrium. Western blotting assays demonstrated that Atp4 deletion led to the sharp drop of Cob1、Cox1、Cox2 and Atp6 protein and the light drop of Cox and Cox4. Sum of above results,Atp4 plays its function in mitochondrion,and it is essential in maintaining the normal expressions of mitochondrial proteins and function of mitochondrial respiratory chain.

Key words: mitochondrial, Atp4, Saccharomyces pombe, respiratory chain

LI Qin, ZHANG Lin-lin, HUANG Ying. Research on the Localization and Function of Atp4 in Schizosaccharomyces pombe[J]. Biotechnology Bulletin, 2019, 35(8): 59-63.

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