Development of a Colloidal Gold Immunochromatographic Test Strip for the Detection of Potato Virus S

doi:10.13560/j.cnki.biotech.bull.1985.2019-0287

Abstract

Abstract: This work aims to develop a colloidal gold immunochromatographic test strip for on-site rapid detection of potato（Solanum tuberosum L.）virus S（PVS）. Colloidal gold solution was prepared by trisodium citrate reduction method,its maximum absorption value was at the wavelength of 524 nm and the particle size was 20-30 nm. In the strip test,colloidal gold-labeled polyclonal sheep antibody against PVS was sprayed on the glass fiber as the detection antibody,and rabbit anti-goat IgG at the control line and anti-PVS at the test line on the nitrocellulose membrane of the test strip was served as the capture antibody. The positive result was easily judged by the presence of a red test line with naked eye within 2 min. The detection was still feasible when the samples was diluted by 10⁴ W/V,and test strip showed no cross effect when tested by other common 5 viruses（PVX,PVY,PLRV,PVA,and PVM）samples. The detection of potato leaves from field by the test strip was consistent with that by ELISA. Test strip is valid for 180 d while stored in dry sealed low-temperature 4℃. The test strip is convenient,simple and rapid in the field detection of PVS,no additional equipment is required,thus applicability and practicability are wide.

Key words: potato virus S, colloidal gold immunochromatography assay, rapid detection, test strip

ZHANG Wei, LI Zhi-xin, FU Chun-jiang, LIU Wei-ping. Development of a Colloidal Gold Immunochromatographic Test Strip for the Detection of Potato Virus S[J]. Biotechnology Bulletin, 2019, 35(12): 184-188.

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