Application in the Detection of Fungal Toxins by Nucleic Acid Aptamer Lateral Flow Chromatography Analysis Technique

doi:10.13560/j.cnki.biotech.bull.1985.2019-0830

Abstract

Abstract: Lateral flow chromatography analysis technique（LFAs）characterized as simple,rapid,economic,and efficient one and can quickly presents the test results under the laboratory condition. New chemical antibody,nucleic acid aptamer body becomes a novel identifying component because of its in vitro screening,easy synthesis and modification,small batch difference and so on. Aptamer lateral flow chromatography analysis technique is the novel and fast detection technique of combing lateral flow tomography technology with aptamer. The LFAs is mainly used for qualitative,semi-quantitative and quantitative detection of contaminants in food safety,environment and clinical analysis. This paper reviews the LFA susing the aptamer combined with markers such as fluorescent,nano-gold and magnetic beads,as well as their applications in the rapid detection of fungal toxins through optics,colorimetry,surface-enhanced raman scattering,which aims at providing a reference in designing aptamer lateral flow chromatography analysis biological sensing technology.

Key words: lateral flow chromatography analysis technique, aptamer, biosensor, rapid detection, fungal toxin

ZHAO Ying, WANG Nan, LU An-xiang, FENG Xiao-yuan, GUO Xiao-jun, LUAN Yun-xia. Application in the Detection of Fungal Toxins by Nucleic Acid Aptamer Lateral Flow Chromatography Analysis Technique[J]. Biotechnology Bulletin, 2020, 36(8): 217-227.

References

[1] Wang C, Sun LL, Zhao Q.A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1[J]. Chinese Chemical Letters, 2019, 30(5):1017-1020.
[2] 陈尔凝, 赵新颖, 屈锋. 细菌的核酸适配体筛选的研究进展[J]. 色谱, 2016, 34(4):389-396.
Chen RN, Zhao XY, Qu F.Research advances of aptamers selection for bacterium targets[J]. Chinese Journal of Chromatography, 2016, 34(4):389-396.
[3] Wang Y, Li S, You Y, et al.Application progress of aptamer in biotoxin detection[J]. Journal of Henan Agricultural Sciences, 2018, 47(2):1-6.
[4] Liu Y, Cai Z, Sheng L, et al.A magnetic relaxation switching and visual dual-mode sensor for selective detection of Hg²+ based on aptamers modified Au@Fe₃O₄nanoparticles[J]. Journal of Hazardous Materials, 2019, 388:121728.
[5] Yang X, Kong W, Yang M, et al.Application of aptamer identification technology in rapid analysis of mycotoxins[J]. Chinese Journal of Analytical Chemistry, 2013, 41(2):297-306.
[6] Van-thuan N, Young S, Man B. Aptamer-based environmental biosensors for small molecule contaminants[J]. Current Opinion in Biotechnology, 2017, 45:15-23.
[7] Xie Y, Yang Y, Kong W, et al.Application of nanoparticle probe-based lateral flow immunochromatography technology in mycotoxins detection[J]. Chin J Anal Chem, 2015, 43(4):618-628.
[8] Li Y, Sun L, Zhao Q.Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA[J]. Anal Bioanal Chem, 2018, 410(24):6269-6277.
[9] 张勋. 真菌毒素类高灵敏高通量快速检测方法研究[D]. 无锡:江南大学, 2014.
Zhang X.High-sensitivity, high-throughput and rapid detection immunoassay for mycotoxins[J]. Wuxi:Jiangnan University, 2014.
[10] Wang L, Ma W, Chen W, et al.An aptamer-based chromatographic strip assay for sensitive toxin semi-quantitative detection[J]. Biosens Bioelectron, 2011, 26(6):3059-3062.
[11] 郑睿行, 周子焱, 傅晓, 等. HPLC测定玉米中玉米赤霉烯酮和赭曲霉毒素A[J]. 食品研究与开发, 2017, 38(19):139-142.
Zheng RX, Zhou ZY, Fu X, et al.Determination of zearalenone and ochratoxin A in corn by means of HPLC[J]. Food Research and Development, 2017, 38(19):139-142.
[12] Michele H, Elisa WJ, Beatriz NI.Photometric procedure for quantitative analysis of aflatoxin B1 in peanuts by thin-layer chromatography using charge coupled device detector[J]. Quimia Nova, 2010, 33(1):43-47.
[13] 张春娥, 刘新保, 廖若宇, 等. 高效液相色谱法测定全麦粉中呕吐毒素含量[J]. 粮油食品科技, 2018, 26(5):53-57.
Zhang CE, Liu XB, Liao RY,et al.Determination of the contents of vomiting toxin(DON)in whole wheat flour by HPLC[J]. Science and Technology of Cereals, Oils and Foods, 2018, 26(5):53-57.
[14] Zhao C, Ban X, Liu S, et al.Determination of AFB1 in corns by high performance liquid chromatography without derivations[J]. Journal of Food Safety & Quality, 2014, 5(7):2198-2203.
[15] 贾海飞, 刘修恒, 吴拥军, 等. 黄曲霉毒素B₁胶体金免疫层析法检测试纸条的制备[J]. 职业与健康, 2016, 32(11):1495-1501.
Jia HF, Liu XH, Wu YJ, et al.Preparation of colloidal gold immunochromatographic test strip fpr aflatoxin B1[J]. Occupation and Health, 2016, 32(11):1495-1501.
[16] 王一晨, 刘奇, 吴学贵, 等. 高效液相色谱-串联质谱法测定花生中的黄曲霉毒素B₁[J]. 食品安全质量检测学报, 2019, 10(7):2059-2063.
Wang YC, Liu Q, Wu XG, et al.Determination of aflatoxin B1 in peanut by high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety & Quality, 2019, 10(7):2059-2063.
[17] 李红梅, 朱聿元, 韩华, 等. 高效液相色谱-柱后光化学衍生法测定大米粉中黄曲霉B₁含量[J]. 安徽农学通报, 2017, 23(15):135-137.
Li HM, Zhu YY, Han H, et al.Separation and identification of aflatoxin B1 in rice meal by high performance liquid chromatography with post-column photochemical reaction[J]. Anhui Agricultural Science Bulletin, 2017, 23(15):135-137.
[18] Joseprubert, Carla soler, Jordi manes. Application of an HPLC-MS/MS method for mycotoxin analysis in commercial baby foods[J]. Food Chemistry 2012, 133(1):176-183.
[19] 张桂兰. 基于适配体的赭曲霉毒素和雌激素快速检测方法研究[D]. 北京:中国农业科学院, 2018.
Zhang GL.Development of aptamer-based rapid detection methods for ochratoxin and estrogen[J]. Beijing:Chinese Academy of Agricultural Sciences, 2018.
[20] 易守军, 何盼, 欧宝立, 等. 适配体传感法快速测定啤酒中赭曲霉毒素A[J]. 光谱学与光谱分析, 2019, 39(7):2283-2287.
Yi SJ, He P, Ou BL, et al.A novel aptasensors assay for fast detection of ochratoxin A in beer[J]. Spectroscopy and Spectral Analysis, 2019, 39(7):2283-2287.
[21] Zhang X, He K, Fang Y, et al.Preparation and application of ochratoxin A and gibberellone in quantitative colloidal gold immunochromatography strip[J]. Journal of Zhejiang University-Science Biomedicine & Biotechnology, 2018, 19(11):871-883.
[22] Wang J, Liu B, Hsu Y, et al.Sensitive competitive direct enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip for detecting aflatoxin M1 in milk[J]. Food Control, 2011, 22(6):964-969.
[23] 周伟璐. 胶体金适配子试纸条现场快速筛查中药中赭曲霉毒素A研究[D]. 镇江:江苏大学, 2016.
Zhou WL.An aptamer based colloidal gold tes strip for on-site rapid screening of ochratoxin A in traditional Chinese medicines[J]. Zhenjiang:Jiangsu University, 2016.
[24] Alina V, Alexandr E, Anatoly V, et al.Gold nanoparticles of different shape for bicolor lateral flow test[J]. Analytical Biochemistry, 2019, 568(1):7-13.
[25] Shim W, Kim M, Mun H, et al.An aptamer-based dipstick assay for the rapid and simple detection of aflatoxin B1[J]. Biosensors and Bioelectronics, 2014, 62:288-294.
[26] Chen L, Wen Fang, Li M, et al.A simple aptamer-based fluorescent assay for the detection of Aflatoxin B1 in infant rice cereal[J]. Food Chemistry 2017, 215(15):377-382.
[27] Kolosova A, Sibanda L, Dumoulin F, et al.Lateral-flow colloidal gold-based immunoassay for the rapid detection of deoxynivalenol with two indicator ranges[J]. Anal Chim Acta, 2008:616, 235-244.
[28] 杨秀梅, 邢国杰, 徐宽, 等. 胶体金免疫层析技术定量检测人绒毛膜促性腺激素[J]. 上海师范大学学报:自然科学版, 2012, 41(3):274-279.
Yang XM, Xing GJ, Xu K, et al.Quantitative detection of human chorionic gonadotrophin using colloid gold immunochromatographic assay[J]. Journal of Shanghai Normal University Natural Sciences, 2012, 41(3):274-279.
[29] Zhu C, Zhao Y, Yan M, et al.A sandwich dipstick assay for ATP detection based on split aptamer fragments[J]. Analytical &Bioanalytical Chemistry, 2016, 408(15):1-8.
[30] Miriam J, Mohammad S, et al.Advances in aptamers-based lateral flow assays[J]. Trends in Analytical Chemistry, 2017.
[31] 朱超. 基于荧光适配体试纸条的黄曲霉毒素B₁快速检测技术研究[D]. 北京:中国农业科学院, 2017.
Zhu C.Development of aptamer-based lateral flow fluorescent assay for fast screening of aflatoxin B1[J]. Beijing:Chinese Academy of Agricultural Sciences, 2017.
[32] Zhou W, Kong W, Dou X, et al.An aptamer based lateral flow strip for on-site rapid detection of ochratoxin A inAstragalusmembranaceus[J]. Journal of Chromatography B Analytical Technologies in the Biomedical & Life Sciences, 2016, 1022:102-108.
[33] Zhang G, Zhu C, Huang Y, et al.A Lateral flow strip based aptasensor for detection of ochratoxin a in corn samples[J]. Molecules, 2018, 23(2):291.
[34] 周子明, 谢彩玲, 赵斌, 等. 基于分子发夹/荧光微球的侧向层析法定量检测黄曲霉毒素B1[J]. 分析测试学报, 2018, 37(6):689-695.
Zhou ZM, Xie CL, Zhao B, et al.Determination of aflatoxin B1 by lateral flow assay based on molecule beacon/fluorescent microsphere[J]. Journal of Instrumental Analysis, 2018, 37(6):689-695.
[35] 肖理文, 徐秀, 赵皖, 等. 基于时间分辨荧光纳米微球的玉米赤霉烯酮快速定量检测试纸条的研制及性能研究[J]. 粮食加工, 2017, 42(5):37-39.
Xiao LW, Xu X, Zhao W, et al.Development and performance of a fast quantitative detection strip for corn gibberellinone based on time-resolved fluorescent nanospheres[J].Grain Processing,2017, 42(5):37-39.
[36] 沙志聪, 其木格, 贾增艳, 等. 量子点标记免疫层析试纸条检测谷物中赭曲霉毒素A[J]. 食品工业科技, 2019, 40(17):191-195.
Sha ZC, Qi MG, Jia ZY, et al.Quantum dot-labeled immunochrom-atographic test strip for rapid detection ochratoxin A in cereals[J]. Science and Technology of Food Industry, 2019, 40(17):191-195.
[37] Peng J, Huang Q, Liu Y, et al.Photoelectrochemical sensor based on composite of CdTe and nickel tetra-aminedphthalocyanine covalently linked with graphene oxide for ultrasensitive detection of curcumin[J]. Sensors and Actuators B:Chemical, 2019, 294(1):157-165.
[38] Thatyara O, Auro A, Flavio S, et al.Photoelectrochemical determi-nation of tert-butylhydroquinone in edible oil samples employing CdSe/ZnS quantum dots and LiTCNE[J]. Food Chemistry, 2017, 227(15):16-21.
[39] Richarte A, Bruno J.Aptamer-quantum dot lateral flow test strip development for rapid and sensitive detection of pathogenic escherichia coli via intimin, O157- Specific LPS andshiga toxin 1 aptamers[J]. Current Bionanotechnology, 2015, 1(2):80-86.
[40] Wang L, Chen W, Ma W, et al.Fluorescent strip sensor for rapid determination of toxins[J]. Chemical Communications, 2011, 47(5):1574-1576.
[41] Duan H, Li Y, Shao Y, et al.Multicolor quantum dot nanobeads for simultaneous multiplex immunochromatographic detection of mycotoxins in maize[J]. Sensors and Actuators B:Chemical, 2019, 291:411-417.
[42] Yeon S, KimN, Hanun A, et al. Aptamer-based nanobiosensors[J]. Biosensors and Bioelectronics, 2016, 76:2-19.
[43] 栾云霞, 陈佳伟, 陆安祥, 等. 基于非标记核酸适配体可视化检测黄曲霉毒素B₁的方法研究[J]. 食品安全质量检测学报,2014, 5(3):736-741.
Luan Y, Chen J, Lu A, et al.Colorimetric detection of aflatoxin B1 using aptamer-functionalized gold nanoparticles[J]. Journal of Food Safety & Quality, 2014, 5(3):736-741.
[44] Badie B, Danesh N, Karimi G, et al.Ultrasensitive detection of ochratoxin A using aptasensors[J]. Biosensors and Bioelectronics, 2017, 98:168-179.
[45] Wu S, Liu L, Duan N, et al.An aptamer-based lateral flow test strip for rapid detection of zearalenone in corn samples[J]. Journal of Agricultural and Food Chemistry, 2018, 66(8):1949-1954.
[46] Liao J, Li H.Lateral flow immunodipstick for visual detection of aflatoxin B1in food using immuno-nanoparticles composed of a silver core and a gold shell[J]. Microchimica Acta, 2010, 171(3-4):289-295.
[47] Kolosova A, Sibanda L, Dumoulin F, et al.Lateral-flow colloidal gold-based immunoassay for the rapid detection of deoxynivalenol with two indicator ranges[J]. Analytica Chimica Acta, 2008, 616(2):235-244.
[48] 周朴帆, 陈丽丽. 金纳米颗粒的生物检测应用[J]. 中国生物制品学杂志, 2018, 31(7):792-796.
Zhou PF, Chen LL.Application of gold nanoparticles in biological detection[J]. Chinese Journal of Biologicals, 2018, 31(7):792-796.
[49] Slowing I, Trewyn B, Giri S, et al.Mesoporous silica nanoparticles for drug delivery and biosensing applications[J]. Advanced Functional Materials, 2007, 17(8):1225-1236.
[50] Huang H, Zhu J.DNA aptamer-based QDs electrochemilumines-cence biosensor for the detection of thrombin[J]. Biosensors & Bioelectronics, 2009, 25(4):927-930.
[51] Li W, Dong K, Wang H, et al.Remote and reversible control of in vivo bacteria clustering by NIR-driven multivalent upconverting nanosystems[J]. Biomaterials, 2019, 217:119310.
[52] 颜范勇, 邹宇, 王猛, 等. 荧光碳点的制备及应用[J]. 化学进展, 2014, 26(1):61-74.
Yan FY, Zhou Y, Wang M, et al.Synthesis and application of the fluorescent carbon dots[J]. Progress in Chemistry, 2014, 26(1):61-74.
[53] 荣杰峰, 许美珠, 吴洪成, 等. 基于石墨烯量子点的检测地表水中六价铬的荧光传感器[J]. 分析试验室, 2017(1):57-61.
Rong JF, Xu MZ, Wu HC, et al.Fluorescent sensor for chromium(VI)in surface water based on graphene quantum dots[J]. Chines Journal of Analysis Laboratory, 2017(1):57-61.
[54] Eddaoudi M, Kim J, Rosi N, et al.Systematic design of pore size and functionality in isoreticular MOFs and their application in methane storage[J]. Science, 2002, 295(5554):469-472.
[55] Zhang W, Xiao X, Liu X, et al.Preparation and quality of SERS test strips[J]. Chemical Journal of Chinese Universities, 2013, 34(6):1385-1388.
[56] Jia X, Wang C, Zhen R, et al.Dual dye-loaded Au@Ag coupled to a lateral flow immunoassay for the accurate and sensitive detection of Mycoplasma pneumoniaeinfection[J]. RSC Advances, 2018, 8(38):21243-21251.
[57] Kalachyova Y, Lyutakov O, Kostejn M, et al.Silver nanostructures:From individual dots to coupled strips for the tailoring of SERS excitation wavelength from near-UV to near-IR[J]. Electronic Materials Letters, 2015, 11(2):288-294.
[58] Li A, Tang L, Song D, et al.A SERS-active sensor based on heterogeneous gold nanostar core-silver nanoparticle satellite assemblies for ultrasensitive detection of aflatoxinB1[J]. Nanoscale, 2016, 8(4):1873.
[59] Restaino SM, White IM.A critical review of flexible and porous SERS sensors for analytical chemistry at the point-of-sample[J]. Analytica Chimica Acta, 2019(1060):17-29.
[60] Kim H, Awofeso O, Choi S M, et al.Colorimetric analysis of saliva-alcohol test strips by smartphone-based instruments using machine-learning algorithms[J]. Applied Optics, 2017, 56(1):84.
[61] Wei X, Meng X, Fu Q, et al.A portable smart-phone readout device for the detection of mercury contamination based on an aptamer-assay nanosensor[J]. Sensors, 2016, 16(11):1871.
[62] Jin B, Yang YX, He RY, et al.Lateral flow aptamer assay integrated smartphone-based portable device for simultaneous detection of multiple targets using up conversion nanoparticles[J]. Sensors and Actuators B:Chemical, 2018, 276(10):48-56.