Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (11): 245-258.doi: 10.13560/j.cnki.biotech.bull.1985.2019-1273

Previous Articles     Next Articles

Research Progress of DNA Aptasensors for Foodborne Pathogen Detection

WANG Qi1(), YAN Chun-lei1, GAO Hong-wei2, WU Wei1(), YANG Qing-li1()   

  1. 1. College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109
    2. Electromechanic Engineering College,Qingdao Agricultural University,Qingdao 266109
  • Received:2019-12-29 Online:2020-11-26 Published:2020-11-20
  • Contact: WU Wei,YANG Qing-li E-mail:17854231141@163.com;wuweiouc@126.com;rice407@163.com

Abstract:

Aptamers are single-stranded DNA or RNA molecules which possess specific identification and binding ability to their targets. Aptamers present breathtaking potential in the field of foodborne pathogen detection and other bio-molecular recognition,owing to their excellent specificity,stabilization,selectivity,simplicity of modification,and high affinity. This review outlines the technologies of aptamer screening and aptamer application,including colorimetric assays,visible spectrum assay,fluorescence assays,electrochemical assays,surface-enhanced Raman assays,and gold-nanoparticle lateral flow assays in the determination of foodborne pathogens. Until now,the remarkable progress(more rapid,sensitive,and accurate)in the monitoring of foodborne pathogen on aptasensor has been achieved;however,there are still many challenges and flaws in their utilization to be overcome.

Key words: aptamer, food safety, rapid detection, SELEX, Escherichia coli, Salmonella