Cloning,Sequence Analysis and Expression Characteristics of Gene Ty-6 Related to Tomato Resistance to Yellow Leaf Curl Disease

doi:10.13560/j.cnki.biotech.bull.1985.2019-1042

Abstract

Abstract: This work aims to further explore the yellow leaf curl disease of tomato at the molecular level and expand its disease resistance gene pool. The experiment was carried out by cloning the gene Ty-6 related to tomato yellow leaf curl disease,then using bioinformatics to conduct sequence analysis of its primary,secondary and tertiary structures,further constructing a phylogenetic tree,and finally detecting the expression characteristics of the gene Ty-6 in different tissues at different stages after infecting yellow leaf curl virus. The results showed that the gene Ty-6 encoded 305 amino acids,the encoded product belonged to the basic protein,and the secondary structure was dominated by random coils. The protein was predicted to be localized on the plasma membrane;and it contained 51 phosphorylation sites and 39 glycosylation sites. The promoter region was mainly TATA-box,and contained multiple elements of light response,jasmonic acid,salicylic acid and abscisic acid to induce gene expression. Additionally,phylogenetic tree results indicated that its affinity relation with potato was the closest. q-PCR results demonstrated that the expression level of this gene in the leaves after infection was significantly higher than that of the control group,and it expressed tissue specially. The results of studying gene Ty-6 not only expand Ty gene toolkit availability to breeders,and also provide a feasible approach of resisting disease.

Key words: tomato, yellow leaf curl disease, Ty-6, bioinformatics, expression characteristics

ZHU Xiao-lin, WEI Xiao-hong, WANG Bao-qiang, WANG Xian, ZHANG Chao-yang. Cloning,Sequence Analysis and Expression Characteristics of Gene Ty-6 Related to Tomato Resistance to Yellow Leaf Curl Disease[J]. Biotechnology Bulletin, 2020, 36(7): 40-47.

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