Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (8): 81-87.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0217

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Isolation and Identification of Pathogenic Fungus of Botrytis cinerea and Screening of Antagonistic Bacteria Against Tomato Gray Mold

CHEN Zhe, HUANG Jing, ZHAO Jia, LIANG Hong   

  1. Biotechnology Research Center,Shanxi Academy of Agriculture Science,Taiyuan 030031
  • Received:2017-03-21 Online:2017-08-01 Published:2017-08-01

Abstract:

It is well known that Botrytis cinerea is one of the most geographically widespread plant pathogen on tomato. A fungus BC2016-2 was successfully isolated from the tomato surface and identified as B. cinerea,a pathogen of tomato gray mold. Then,three strains with obvious inhibitory effects were screened using B. cinerea BC2016-2 as indicator fungus,and they were Bacillus amyloliquefacien CM3,Bacillus megaterium Y-30 and Bacillus amyloliquefaciens Y-48. The greenhouse pot experiment showed that the control effects of CM3,Y-30 and Y-48 on the tomato gray mold caused by B. cinerea BC2016-2 were 65.58%,54.1% and 72.13%,respectively.

Key words: Botrytis cinerea, Bacillus amyloliquefaciens, Bacillus megaterium, biological control