Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (10): 105-115.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0282

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Screening of an Effective Sulfur-oxidizing Strain and Its Main Bio-oxidation Metabolic Pathway of S2-

ZHANG Lin-yi1,2(), SONG Chen3, XU Yao-yao2, WANG Jia-ning2, WANG Jin1, YUE Zheng-bo1, LIU Xiao-ling2()   

  1. 1. School of Resources and Environmental Engineering,Hefei University of Technology,Hefei 230009
    2. Chinese Research Academy of Environmental Sciences,Beijing 100012
    3. Nanjing Frontier Environmental Technology Co. Ltd.,Nanjing 210029
  • Received:2020-03-16 Online:2020-10-26 Published:2020-11-02
  • Contact: LIU Xiao-ling E-mail:506739981@qq.com;liuxl@craes.org.cn

Abstract:

Inorganic sulfur(S2-)was used as the target pollutant,and an indigenous microorganism capable of efficient S2--oxidizing was isolated from Dongsha river,a black-odor water body in Beijing. Through 16S rRNA sequencing experiment,this strain was identified as Citrobacter and named as Citrobacter sp.sp1. The optimal S 2--oxidizing conditions of this strain sp1 were as follows:25℃,initial pH of 7.0,and initial glucose concentration of 1.00 g/L along with initial cell concentration of 1.00 g/L. Under the above optimal conditions,the highest S2--oxidizing ratio of artificial sulfur-containing wastewater by this strain sp1 reached 97.1%. Five chemical forms of sulfur including S0、S2O32-、SO32-、S4O62- and SO42- were produced in the whole bio-reaction process,and the concentration of SO42- increased slowly with the decreasing of S2-. Results of high-throughput sequencing showed that the strain sp1. transformed the unsteady-state S2-into steady-state SO42-via two main S2--oxidizing metabolic pathways of Paracoccus sulfur oxidation(PSO)and S4 intermediate(S4I). In PSO pathway,S2- partially was oxidized to be S0 and then the generated S0 continuously was oxidized to be SO32,while other part of S2- were directly bio-converted to SO32,and intermediate SO32- were further oxidized to SO42- by both directly and indirectly oxidation pathways;in addition,S0 reacted with SO32- spontaneously to form S2O32-,while S2O32- released SO32- and S0 by the disproportionate reaction. In S4I pathway,S2O32- partially was oxidized to S4O62-,and S4O62- subsequently was then oxidized to SO42-.

Key words: black-odor water body, S2-, sulfur-oxidizing bacterium, sulfur-oxidizing characteristics, metabolic pathway