Identification of Newly Isolated Xanthobacter sp. and Its Degradability to Phthalic Acid Esters

doi:10.13560/j.cnki.biotech.bull.1985.2018-0393

Abstract

Abstract: Phthalic acid esters（PAEs）are ubiquitous organic pollutants with environmental estrogen effects,which cause serious threats to human health and safety of ecological system. Strain YC-JY1 was isolated from long-time contaminated soil by petroleum,and grew by utilizing dibutyl phthalate（DBP）as the sole carbon source. After 16S rDNA sequencing,it was identified to be a strain of Xanthobacter genus. The optimal conditions for DBP degradation by this strain were 30℃,pH 7.0 and no NaCl added. Under this condition,100 mg/L DBP was completely degraded within 5 d. As the concentration of DBP increased,the degradation rate of it to DBP with initial concentration of 200 - 400 mg/L was over 94% after 5 d. Through substrate spectrum experiments,it was found that strain YC-JY1 utilized extensively other PAEs. The degradation rate of it to dipentyl phthalate（DPeP）and dihexyl phthalate（DHP）were all above 90%. The intermediates of DBP were determined to be mono-butyl phthalate（MBP）and phthalic acid（PA）by HPLC-MS. It was inferred that the initial metabolic process of DBP was as firstly hydrolyzed to MBP and then to PA by the strain YC-JY1.

Key words: Di-n-butyl phthalate, biodegradation, Xanthobacter sp. YC-JY1, metabolic pathway^?

WANG Jia-yi FAN, Shuang-hu, REN Chao, WANG Jun-huan, YANG Ting, JIA Yang, LI Xian-jun, YAN Yan-chun. Identification of Newly Isolated Xanthobacter sp. and Its Degradability to Phthalic Acid Esters[J]. Biotechnology Bulletin, 2018, 34(10): 157-164.

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