Cloning of ZmENA1 and Its Responses to Metal Ions in Maize

doi:10.13560/j.cnki.biotech.bull.1985.2020-0192

Abstract

Abstract:

Nicotianamine（NA）is the precursor of mugineic acid（MA）which is a phytosiderophore of plants,and nicotianamine effluxer（ENA）is an important protein mediating NA transport in plants. Thus,exploring the functions of ZmENA1 may provide bases for understanding metal ions transport and storage mechanisms in maize. In this study,ZmENA1 was homologously cloned and the basic characteristics of the encoded protein were analyzed via bioinformatics. Also the expression patterns of ZmENA1 during kernel development process under different metal ions concentrations were examined using real-time quantitative PCR. The results showed that the ORF of ZmENA1 was 1 356 bp,and the encoded protein had a deduced isoelectric point of 8.06 and molecular weight of 48.998 kD. In addition,ZmENA1 contained 53.98% proportion of α-helix,four phosphorylation sites,10 transmembrane regions,and 1 N terminal signal peptide. The expression of ZmENA1 in the mixture of endosperm and pericarp was higher than that in the embryo,reached a peak at 16 DAP,and decreased with the developmental process. Besides,the expression of ZmENA1 presented fluctuations in response to deficiency of Zn²⁺,Mn²⁺,and Cu²⁺,as well as excess of Zn²⁺,Mn²⁺,and Cd²⁺,suggesting that ZmENA1 played a role in mediating transport and storage of these metal ions. This study may provide a basis for further revealing the function of ZmENA1.

Key words: maize, ZmENA1, metal, nicotianamine, bioinformatics

ZHANG Xin, ZHOU Xiao-jin, PANG Sen, LI Su-zhen, HUANG Jia-xing, CHEN Ru-mei. Cloning of ZmENA1 and Its Responses to Metal Ions in Maize[J]. Biotechnology Bulletin, 2020, 36(11): 1-8.

Figures/Tables 6

References 31

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