Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (1): 24-32.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1383

Previous Articles     Next Articles

Advances in Analysis Methods of Mass Spectrometry-based Metabolomics

TIAN He(), SHUI Guang-hou()   

  1. Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101
  • Received:2020-11-13 Online:2021-01-26 Published:2021-01-15
  • Contact: SHUI Guang-hou E-mail:tianhe@genetics.ac.cn;ghshui@genetics.ac.cn

Abstract:

Mass spectrometry(MS)-based metabolomics analyses are now widely applied to identify and quantify small-molecular metabolites in biological samples for uncovering endogenous alterations in cells after their exposure to exogenous stimulus. Duo to the complicated components in biological samples and a big span of polarities of metabolites ranged from hydrophilic to hydrophobic metabolites,a series of extraction protocols and the matched liquid chromatography(LC)and MS conditions are required to obtain the quantitative and qualitative information of target components. To some extent,the diversity of structure and physicochemical properties of small molecule metabolites limits the high-throughput acquirement of whole metabolic profiles from biological samples. Present review focuses on the introduction and discussion of extraction methods of varied metabolites,as well as their corresponding LC-MS conditions.

Key words: mass spectrometry, metabolomics, lipidomics