Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (1): 24-32.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1383
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Received:
2020-11-13
Online:
2021-01-26
Published:
2021-01-15
Contact:
SHUI Guang-hou
E-mail:tianhe@genetics.ac.cn;ghshui@genetics.ac.cn
TIAN He, SHUI Guang-hou. Advances in Analysis Methods of Mass Spectrometry-based Metabolomics[J]. Biotechnology Bulletin, 2021, 37(1): 24-32.
1H-NMR | GC-MS | LC-MS | |
---|---|---|---|
灵敏度 | nmol/L | amol/L | amol/L |
特异性 | 质子信号易重叠干扰定性、定量 | 强 | 强 |
稳定性 | 无需内标校准 | 依赖内标校准 | 依赖内标校准 |
样品前处理 | 要求低或无需前处理 | 要求高,除蛋白与杂质 | 要求高,除蛋白与杂质 |
代谢组定性数目 | 一般小于100个 | 几千到几万个 | 几千到几万个 |
检测代谢物种类 | 氨基酸、有机酸、碳水化合物等 | 热稳定、易挥发或衍生化后易挥发 | 热不稳定、难挥发 |
分子量 | 动物样品一般小于400,植物样品通常小于800 | 小于1 000 | 分子量没有上限 |
1H-NMR | GC-MS | LC-MS | |
---|---|---|---|
灵敏度 | nmol/L | amol/L | amol/L |
特异性 | 质子信号易重叠干扰定性、定量 | 强 | 强 |
稳定性 | 无需内标校准 | 依赖内标校准 | 依赖内标校准 |
样品前处理 | 要求低或无需前处理 | 要求高,除蛋白与杂质 | 要求高,除蛋白与杂质 |
代谢组定性数目 | 一般小于100个 | 几千到几万个 | 几千到几万个 |
检测代谢物种类 | 氨基酸、有机酸、碳水化合物等 | 热稳定、易挥发或衍生化后易挥发 | 热不稳定、难挥发 |
分子量 | 动物样品一般小于400,植物样品通常小于800 | 小于1 000 | 分子量没有上限 |
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