Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (3): 44-52.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0800
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LI Hong-ye1(), CHEN Li-jiao1, LIU Ming-li1,3, GUO Tian-jie1,3, WANG Dao-ping2, PAN Ying-hong2, ZHAO Ming1,3()
Received:
2020-06-30
Online:
2021-03-26
Published:
2021-04-02
Contact:
ZHAO Ming
E-mail:837356337@qq.com;zhaoming02292002@aliyun.com
LI Hong-ye, CHEN Li-jiao, LIU Ming-li, GUO Tian-jie, WANG Dao-ping, PAN Ying-hong, ZHAO Ming. Cloning and Expression of Tannase Gene Tan2 from Aspergillus niger[J]. Biotechnology Bulletin, 2021, 37(3): 44-52.
Accession | Blast results(NCBI) | ||
---|---|---|---|
Description | Organism | Identity | |
XP_001401809.1 | tannase | Aspergillus niger CBS 513.88 | 100% |
EHA20668.1 | hypothetical protein | Aspergillus niger ATCC 1015 | 99.83% |
GCB23703.1 | tannase | Aspergillus awamori | 99.65% |
RDK47096.1 | tannase and feruloyl esterase | Aspergillus phoenicis ATCC 13157 | 99.48% |
XP_026632695.1 | tannase and feruloyl esterase | Aspergillus welwitschiae | 99.30% |
XP_025515125.1 | tannase and feruloyl esterase | Aspergillus piperis CBS 112811 | 97.91% |
XP_025567423.1 | tannase and feruloyl esterase | Aspergillus vadensis CBS 113365 | 97.91% |
OJZ87444.1 | hypothetical protein | Aspergillus luchuensis CBS 106.47 | 97.74% |
GAA82877.1 | tannase and feruloyl esterase | Aspergillus luchuensis IFO 4308 | 97.56% |
OJJ71084.1 | hypothetical protein | Aspergillus brasiliensis CBS 101740 | 97.56% |
Accession | Blast results(NCBI) | ||
---|---|---|---|
Description | Organism | Identity | |
XP_001401809.1 | tannase | Aspergillus niger CBS 513.88 | 100% |
EHA20668.1 | hypothetical protein | Aspergillus niger ATCC 1015 | 99.83% |
GCB23703.1 | tannase | Aspergillus awamori | 99.65% |
RDK47096.1 | tannase and feruloyl esterase | Aspergillus phoenicis ATCC 13157 | 99.48% |
XP_026632695.1 | tannase and feruloyl esterase | Aspergillus welwitschiae | 99.30% |
XP_025515125.1 | tannase and feruloyl esterase | Aspergillus piperis CBS 112811 | 97.91% |
XP_025567423.1 | tannase and feruloyl esterase | Aspergillus vadensis CBS 113365 | 97.91% |
OJZ87444.1 | hypothetical protein | Aspergillus luchuensis CBS 106.47 | 97.74% |
GAA82877.1 | tannase and feruloyl esterase | Aspergillus luchuensis IFO 4308 | 97.56% |
OJJ71084.1 | hypothetical protein | Aspergillus brasiliensis CBS 101740 | 97.56% |
Name | Accession | Description | Sum PEP Score | Coverage | # Peptides |
---|---|---|---|---|---|
Tannase Tan2 | A0A0G3ZA16 | Carboxylic ester hydrolase OS=Aspergillus niger | 343.26 | 42.52 | 17 |
Name | Accession | Description | Sum PEP Score | Coverage | # Peptides |
---|---|---|---|---|---|
Tannase Tan2 | A0A0G3ZA16 | Carboxylic ester hydrolase OS=Aspergillus niger | 343.26 | 42.52 | 17 |
单宁酶/(U·mL-1) | ΔGA/(mg·mL-1) | PG/(mg·mL-1) | |
---|---|---|---|
样品管 | 1.02±0.06 | 0.0034±0.0002 | 0.0486±0.00115 |
空白管 | — | — | 0.0545±0.00309 |
对照管 | — | — | 0.0527±0.00274 |
单宁酶/(U·mL-1) | ΔGA/(mg·mL-1) | PG/(mg·mL-1) | |
---|---|---|---|
样品管 | 1.02±0.06 | 0.0034±0.0002 | 0.0486±0.00115 |
空白管 | — | — | 0.0545±0.00309 |
对照管 | — | — | 0.0527±0.00274 |
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