Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 117-126.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1392
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WANG Ying1(), CHEN Yong-jing1, SUN Qing-ye1(), YANG Meng-yao1, WU Dun2
Received:
2020-11-16
Online:
2021-06-26
Published:
2021-07-08
Contact:
SUN Qing-ye
E-mail:3022648144@qq.com;sunqingye@ahu.edu.cn
WANG Ying, CHEN Yong-jing, SUN Qing-ye, YANG Meng-yao, WU Dun. Physiological and Biochemical Characteristics of Inquilinus sp. P6-4 Strain and Its Degradation Characteristics for Naphthalene[J]. Biotechnology Bulletin, 2021, 37(6): 117-126.
基因名称 Gene name | 引物序列 Primer sequence(5'-3') | 基因长度 Gene length/bp |
---|---|---|
PAH HBHA nahU S5H CATA C230 GDO | GAGATGCATACCACGTKGGTTGGA AGCTGTTGTTCGGGAAGAYWGTGCMGTT ATGTGGGTGAAGATGGACCTGCC GCATATCATCGGAGATGGCTTTGGC CAAATACCTCGGTTGCAGCG CCCGAACTGGGCAATACCTT TGATCGACTTCAAAGTGTATTTCGA CAAGCGGTGCAACATCGACC ACVCCVCGHACCATYGAAGG CGSGTNGCAWANGCAAAGT AAGAGGCATGGGGGCGCACCGGTTCGATCA CCAGCAAACACCTCGTTGCGGTTGCC ACGAACGGGATGTCGAGG ACGAAACCGATCAGCCGA | 306 400 112 490 470 298 489 |
Table 1 Primers of key enzyme gene
基因名称 Gene name | 引物序列 Primer sequence(5'-3') | 基因长度 Gene length/bp |
---|---|---|
PAH HBHA nahU S5H CATA C230 GDO | GAGATGCATACCACGTKGGTTGGA AGCTGTTGTTCGGGAAGAYWGTGCMGTT ATGTGGGTGAAGATGGACCTGCC GCATATCATCGGAGATGGCTTTGGC CAAATACCTCGGTTGCAGCG CCCGAACTGGGCAATACCTT TGATCGACTTCAAAGTGTATTTCGA CAAGCGGTGCAACATCGACC ACVCCVCGHACCATYGAAGG CGSGTNGCAWANGCAAAGT AAGAGGCATGGGGGCGCACCGGTTCGATCA CCAGCAAACACCTCGTTGCGGTTGCC ACGAACGGGATGTCGAGG ACGAAACCGATCAGCCGA | 306 400 112 490 470 298 489 |
生化反应 Physiological and biochemical reaction | 结果 Result | 生化反应 Physiological and biochemical reaction | 结果 Result | |
---|---|---|---|---|
1%、2%、3% NaCl生长 1%,2%,3% NaCl growth | + | 固氮 Nitrogen fixation | + | |
过氧化氢酶 Catalase | + | 柠檬酸盐 Utilization of citrate | + | |
吲哚乙酸 Indole acetic acid | + | VP试验 VP test | - | |
有机磷 Organophosphorus | + | 厌氧生长 Anaerobic growth | - | |
无机磷 Inorganic phosphorus | + | 淀粉酶 Amylase | - | |
革兰氏染色 Gram staining | - | 铁载体 Iron carrier | + |
Table 2 Physiological and biochemical characteristics of P6-4 degrading strain
生化反应 Physiological and biochemical reaction | 结果 Result | 生化反应 Physiological and biochemical reaction | 结果 Result | |
---|---|---|---|---|
1%、2%、3% NaCl生长 1%,2%,3% NaCl growth | + | 固氮 Nitrogen fixation | + | |
过氧化氢酶 Catalase | + | 柠檬酸盐 Utilization of citrate | + | |
吲哚乙酸 Indole acetic acid | + | VP试验 VP test | - | |
有机磷 Organophosphorus | + | 厌氧生长 Anaerobic growth | - | |
无机磷 Inorganic phosphorus | + | 淀粉酶 Amylase | - | |
革兰氏染色 Gram staining | - | 铁载体 Iron carrier | + |
Fig.5 PCR amplified electrophoretic map of key enzyme genes M:Molecular weight standard. 1:PAH gene fragment. 2:HBHA gene fragment. 3:nahU gene fragment. 4:S5H gene fragment. 5:CATA gene fragment. 6:C230 gene fragment. 7:GDO gene fragment
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