Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 58-65.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1267

Previous Articles     Next Articles

Inhibitory Effect of Resveratrol on Walnut Bacterial Black Spot Pathogen

LI Feng1(), CHEN Wen-wen1, DENG Jiang-li1, MAO Qing-li2, QUAN Wen-li1, MAO Ya-hui1()   

  1. 1. Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables/College of Life Science and Technology,Hubei Engineering University,Xiaogan 432000
    2. College of Food Science and Technology,Wuhan University of Bioengineering,Wuhan 430415
  • Received:2020-10-15 Online:2021-06-26 Published:2021-07-08
  • Contact: MAO Ya-hui E-mail:1023905224@qq.com;maoyh3@sina.com

Abstract:

It aims to provide theoretical basis for the development of natural bactericide for walnut bacterial black spot,and to lay a foundation for the application of resveratrol in the prevention and control of agricultural and forestry crop diseases. The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of resveratrol against Xanthomonas arboricola pv. juglandis(Xaj)were determined. The following experiments were carried out under the sub-inhibitory concentration of resveratrol:1)The formation of Xaj biofilm was detected by crystal violet staining. 2)The production of exopolysaccharide(EPS)was determined. 3)The secretion of extracellular enzyme was analyzed on the corresponding solid medium by biochemical method. 4)The motility of Xaj was detected on swarming medium. The results revealed that the MIC of resveratrol against Xaj was 20 μg/mL,and MBC was 45 μg/mL. The formation of Xaj biofilm was effectively inhibited by resveratrol at a sub-inhibitory concentration of 15 μg/mL. Under this concentration,the production of EPS was also significantly inhibited. Resveratrol effectively inhibited the secretion of Xaj protease,promoted the secretion of amylase,but had no effect on the secretion of cellulase. There was no effect on motility(swarming). Resveratrol has an inhibitory effect on walnut bacterial black spot pathogen,affecting the virulence factors of this bacterium,and is expected to develop a suitable plant-derived fungicide for the prevention and treatment of walnut black spot disease.

Key words: resveratrol, walnut bacterial black spot pathogen, antibacterial effect, botanical fungicides