Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 66-72.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0473

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Screening and Identification of an Antagonist Against the Pathogen of Kiwifruit Canker and Its Antifungal Activity to the Phytopathogenic Fungus

ZHU Hai-yun(), MA Yu(), KE Yang, LI Bo   

  1. Shaanxi Microbiology Institute,Xi'an 710043
  • Received:2020-04-22 Online:2021-06-26 Published:2021-07-08
  • Contact: MA Yu E-mail:zhuhaiyun@ms.xab.ac.cn;wefly@ms.xab.ac.cn

Abstract:

The objective is to screen and identify the endophytic bacteria from wild Ginkgo biloba against the pathogen of kiwifruit canker,and to determine the bacteriostatic spectrum of common plant fungal diseases. Three-step method was used to isolate the endophytic bacteria from G. blioba. And then the Pseudomonas syringae pv.Actinidiae(Psa)was used as indicator bacterium for screening the antagonistic bacteria. Combination of morphological,physiological and biochemical characteristics and 16S rRNA sequence analysis was used to identify the antagonistic bacteria. Finally,the pot experiment was carried out to study its control efficacy against kiwifruit canker,and the confrontation method was used to evaluate the antifungal activity of the strain. Total 7 endophytic bacterial strains with antagonistic activities to Psa were obtained. The strain MA23 demonstrated the greatest antagonistic activity toward Psa and was identified as Bacillus cereus. The control efficacy of MA23 fermentation broth supernatant against kiwifruit canker was 93.6%. Meanwhile,MA23 had great capability of antagonism to some plant pathogenic fungi. In conclusion,endophytic bacterium B. cereus MA23 isolated from wild G. biloba not only has good antagonistic activity against the pathogen of kiwifruit canker,but also has significant inhibitory effect on a variety of plant pathogenic fungi.

Key words: endophytic bacteria, kiwifruit canker, Bacillus cereus, antifungal activity