Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 85-96.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1174

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Screening and Genome Sequencing of Cellulytic Bacterium Raoultella ornithinolytica LL1

TANG Hao(), SUN Can, LI Yuan-qiu, LUO Chao-bing()   

  1. College of Life Science,Leshan Normal University,Leshan 614000
  • Received:2020-09-18 Online:2021-06-26 Published:2021-07-08
  • Contact: LUO Chao-bing E-mail:tanghao89@aliyun.com;13366181512@163.com

Abstract:

As a resource-rich natural polymer,cellulose can be degraded into soluble sugar by microorganism. Therefore,screening of efficient cellulose-degrading microorganism has attracted much attention. In the present study,CMC selective medium was used to screen a cellulose-degrading bacterium Raoultella ornithinolytica LL1.The genome sequence of R. ornithinolytica LL1 was conducted by Pacbio Sequl V3 and Illumina Novaseq platform. Furthermore,the cellulase activities of the strain were determined by DNS method. The results showed that the genome size of R. ornithinolytica LL1 was 5 584 354 bp and contained 5 512 genes,of which 5 511,4 419,4 584,4 745,3 610,3 336 and 128 genes were annotated in NR,Swiss-Prot,Pfam,COG,GO,KEGG and CAZyme databases. The genes related to lignocellulose degrading in the genome were also annotated. R. ornithinolytica LL1 presented high cellulase activities,among which β-glucosidase showed the highest activity,followed by endoglucanase and exoglucanase. In this study,the cellulose-degrading ability of R. ornithinolytica LL1 was revealed by genome sequencing and the determination of cellulase activity,which laid a foundation for further cellulose degradation and bioconversion.

Key words: Raoultella ornithinolytica LL1, cellulose degradation, genome sequencing, cellulase