Transcriptome Different Analysis of Tremella aurantialba at Mycelium and Fruiting Body Stages

doi:10.13560/j.cnki.biotech.bull.1985.2020-1038

Abstract

Abstract:

To better understand the gene expression changes of mycelium and fruiting body stages in Tremella aurantialba,high-throughout sequencing were adopted to investigate differentially expressed genes by using RNA sequencing approach. Analysis revealed that 10 992 genes presented differences in gene expression,9 988 genes were up-regulated and 1 004 genes were down-regulated during the fruiting body stage. The Gene Ontology classification showed that the differentially expressed genes were mostly enriched in catalytic activity,binding,cellular process,metabolic process,cell and cell parts. KEGG pathway analysis revealed that the differential expressed genes were involved in three metabolic pathways of amino acid metabolism,carbohydrate metabolism and lipid metabolism. Most genes related to the three metabolic pathways were up-regulated,suggesting that a series of metabolic reactions and other co-regulation were needed during the development of T. aurantialba fruiting body,and these genes may play an important role in the development of T. aurantialba fruiting body. In this study,a lot of gene data were obtained by analyzing transcriptional expressions in T. aurantialba at mycelium and fruiting body stages,and may provide important basis in further revealing the developmental mechanisms and functional genes of T. aurantialba.

Key words: Tremella aurantialba, transcriptome, differentially expressed gene, GO function, pathway analysis

CHEN Zheng-qi, YU Jin-feng, FENG Yun-li, MA Ming, YUE Wan-song, GUO Xiang. Transcriptome Different Analysis of Tremella aurantialba at Mycelium and Fruiting Body Stages[J]. Biotechnology Bulletin, 2021, 37(6): 73-84.

Figures/Tables 14

References 44

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时期 Stage	样品编号 Sample No.	RNA浓度 RNA concentration/(μg·μL^-1)	A₂₆₀/A₂₈₀	A₂₆₀/A₂₃₀	RNA完整度 RNA integrity number (RIN)
菌丝体期 Mycelium stage	TH1	1 216.10	2.09	1.92	9.00
	TH2	812.70	2.09	2.00	9.80
	TH3	837.80	2.06	1.43	8.80
子实体期 Fruiting body stage	TS1	992.70	2.09	1.89	9.60
	TS2	559.00	2.10	1.63	9.70
	TS3	633.10	1.96	1.56	9.60

时期 Stage	样品编号 Sample No.	RNA浓度 RNA concentration/(μg·μL^-1)	A₂₆₀/A₂₈₀	A₂₆₀/A₂₃₀	RNA完整度 RNA integrity number (RIN)
菌丝体期 Mycelium stage	TH1	1 216.10	2.09	1.92	9.00
	TH2	812.70	2.09	2.00	9.80
	TH3	837.80	2.06	1.43	8.80
子实体期 Fruiting body stage	TS1	992.70	2.09	1.89	9.60
	TS2	559.00	2.10	1.63	9.70
	TS3	633.10	1.96	1.56	9.60

Sample	Clean reads	Clean bases	Error rate/%	Q20/%	Q30/%	GC content/%
TH1	34 048 936	5 075 868 853	0.0235	98.68	95.69	53.66
TH2	37 112 666	5 555 268 826	0.0235	98.68	95.66	53.96
TH3	32 225 668	4 822 316 713	0.0236	98.61	95.48	53.93
TS1	55 619 412	8 333 444 436	0.0231	98.82	96.06	56.84
TS2	45 348 528	6 790 586 173	0.0232	98.75	95.94	56.37
TS3	49 513 414	7 408 302 390	0.0236	98.64	95.58	56.22

Sample	Clean reads	Clean bases	Error rate/%	Q20/%	Q30/%	GC content/%
TH1	34 048 936	5 075 868 853	0.0235	98.68	95.69	53.66
TH2	37 112 666	5 555 268 826	0.0235	98.68	95.66	53.96
TH3	32 225 668	4 822 316 713	0.0236	98.61	95.48	53.93
TS1	55 619 412	8 333 444 436	0.0231	98.82	96.06	56.84
TS2	45 348 528	6 790 586 173	0.0232	98.75	95.94	56.37
TS3	49 513 414	7 408 302 390	0.0236	98.64	95.58	56.22

序列长度 Sequence length/bp	序列数量 Number of sequence	百分率 Percentage/%
0-500	44 956	57
501-1 000	13 467	17
1 001-1 500	6 068	8
1 501-2 000	4 049	5
2 001-2 500	2 760	4
2 501-3 000	1 809	2
3 001-3 500	1 331	2
3 501-4 000	976	1
4 001-4 500	679	1
>4 500	2 106	3