Comparison of Molecular Characteristics of the Hybrid Progenies from Different Haplotypes of Ditylenchus destructor

doi:10.13560/j.cnki.biotech.bull.1985.2021-0576

Abstract

Abstract:

In order to clarify whether there are reproductive isolation and the variation in the hybrid offspring between different haplotypes,1 of 3^rd to 4^th stage juveniles and 5 males from different haplotypes were selected to hybridize,and the nematode amounts,ITS-rDNA sequences and secondary structures of F1 generations were analyzed. The results showed that the nematode amount of F1 generations reproduced after hybridized were generally low,and F1 amount of the different cross groups were ≤ 6 per treatment. In some groups,only one nematode was found in one treatment. Due to the low amount of offspring,the ITS-rDNA sequences of 5 F1 generations among 11 groups were successfully sequenced. The ITS-rDNA sequence of F1 generations were different with 0-86 bp from their parents,and all of the secondary structures of ITS were different from those of the parents. The results of ribosomal ITS-RFLP showed diversiform. Phylogenetic analysis showed that some of the F1 generation groups were closer to the male parents,and some were closer to the female parents. No reproductive isolation was found in different haplotypes,but variation appeared mainly in the H9 helix region of ITS1 region in the F1 generation of hybrids,and the degree of variation was directly proportional to the differences between parents. The analysis of differentiation on hybrid generations from different haplotypes of D. destructor were conducted through simulation tests after population diffusions. The results may provide references both to further researches on genetic diversity and control practice of D. destructor.

Key words: Ditylenchus destructor, haplotype, hybridization, RNA secondary structure

NI Chun-hui, LI Hui-xia, LI Wen-hao, LIU Yong-gang, XU Xue-fen, HAN Bian. Comparison of Molecular Characteristics of the Hybrid Progenies from Different Haplotypes of Ditylenchus destructor[J]. Biotechnology Bulletin, 2021, 37(7): 118-126.

Figures/Tables 8

References 27

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序号 Serial number	线虫编号 Sample No.	寄主 Host	采集地 Collecting location	基因型 Haplotype
1	DXP1	马铃薯	甘肃省定西市安定区	B
2	DXP2	马铃薯	甘肃省定西市安定区	C
3	HLJP1	马铃薯	黑龙江绥化市	C
4	MXA2	当归	甘肃省定西市岷县	N
5	TCC1	党参	甘肃省定西市宕昌县	B
6	ZXA1	当归	甘肃省定西市漳县	H
7	WYA4	党参	甘肃省定西市渭源县	K

序号 Serial number	线虫编号 Sample No.	寄主 Host	采集地 Collecting location	基因型 Haplotype
1	DXP1	马铃薯	甘肃省定西市安定区	B
2	DXP2	马铃薯	甘肃省定西市安定区	C
3	HLJP1	马铃薯	黑龙江绥化市	C
4	MXA2	当归	甘肃省定西市岷县	N
5	TCC1	党参	甘肃省定西市宕昌县	B
6	ZXA1	当归	甘肃省定西市漳县	H
7	WYA4	党参	甘肃省定西市渭源县	K

亲本（Parent）♀	亲本（Parent）♂
亲本（Parent）♀	DXP1	DXP2	MXA2	TCC1	ZXA1	WYA4
DXP1	⊕	×	Φ	×	Φ	×
DXP2	×	Φ	×	Φ	Φ	Φ
MXA2	×	×	Φ	Φ	×	Φ
TCC1	Φ	Φ	Φ	Φ	Φ	Φ
ZXA1	Φ	Φ	×	Φ	Φ	Φ
HLJP1	×	Φ	Φ	Φ	Φ	Φ

亲本（Parent）♀	亲本（Parent）♂
亲本（Parent）♀	DXP1	DXP2	MXA2	TCC1	ZXA1	WYA4
DXP1	⊕	×	Φ	×	Φ	×
DXP2	×	Φ	×	Φ	Φ	Φ
MXA2	×	×	Φ	Φ	×	Φ
TCC1	Φ	Φ	Φ	Φ	Φ	Φ
ZXA1	Φ	Φ	×	Φ	Φ	Φ
HLJP1	×	Φ	Φ	Φ	Φ	Φ

杂交组合（父本×母本） Hybridized combination （Male parent× Female parent）	重复管数 Number of repetitions	产生后代杂交管数 Number of progeny-produced tubes	平均后代数（条/管） Average number of offspring （lines per tube）
DXP1×DXP1	20	5	18±2.7b
DXP2×DXP1	20	5	6±1.7b
DXP1×DXP2	20	3	2±0.6b
WYA4×DXP1	20	2	1±0b
DXP1× HLJP1	20	1	1±0b
MXA2×ZXA1	20	5	1±0b
ZXA1×MXA2	20	4	4±1.0b
MXA2×DXP2	20	2	1±0b
DXP2×MXA2	20	3	1±0b
TCC1×DXP1	20	6	103±79.5a
TCC1×HLJP1	20	2	3±1.0b