Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (7): 127-136.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0454

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Optimization of Fermentation Conditions of Myrothecium verrucaria ZW-2,a Biocontrol Strain Against Heterodera glycines and Analysis of Active Substances

CHEN Qian(), ZHANG Lu-yuan, CHEN Bo-chang, WU Hai-yan()   

  1. Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety,Agricultural College of Guangxi University,Nanning 530004
  • Received:2021-04-13 Online:2021-07-26 Published:2021-08-13
  • Contact: WU Hai-yan E-mail:1451208773@qq.com;wuhy@gxu.edu.cn

Abstract:

Myrothecium verrucaria ZW-2 strain had obvious nematicidal activity against Heterodera glycines. In order to determine the optimal fermentation conditions and metabolites of the strain,the orthogonal experiment with four factors and three levels was used to optimize the fermentation conditions and analyze the metabolomics. The results showed that the strain ZW-2 was cultured on Czapek medium with the optimal culture temperature of 28℃,rotation speed of 180 r/min,bottle volume of 100 mL,medium pH of 8,and the optimal carbon and nitrogen sources were sucrose and sodium nitrate. After metabolomics analysis,20 differential metabolites,including amide compounds and aliphatic compounds,were screened according to the information of the charge ratio of metabolites,the accurate molecular weight and the score of first-order fragment ions. The above experiment results by strain ZW-2 further provide important theoretical basis and application value for the development and utilization of this strain.

Key words: Myrothecium verrucaria, Heterodera glycines, optimization of fermentation conditions, metabonomics analysis