Study on the Detoxification Characteristics of Antifungal Lactic Acid Bacteria and the Application of Silage

doi:10.13560/j.cnki.biotech.bull.1985.2021-0897

Abstract

Abstract:

This study aims to add Lactobacillus plantarum to inhibit the proliferation of silage mold and degrade toxins in order to improve feed safety. HPLC was adapted to determine the antibacterial components in the cell-free supernatant（CFS）of L. plantarum and to evaluate the adsorption efficiency of AFB1. After that,L. plantarum and Aspergillus flavus were added to the whole corn（Zea mays L.）silage,the silage at 0,24 and 48 d of fermentation was evaluated by sensory,and the fermentation quality,nutritional value,microorganism,aerobic stability and feed mildew condition were determined and analyzed. The results showed that there were organic acids in CFS,and the adsorption rate of AFB1 by L. plantarum reached 59.40%. The sensory quality,microbial composition,fermentation performance,nutrient composition,feed value,CNCPS,aerobic stability,and feed mildew of the silage treated with L. plantarum were significantly better than those of the control group and the A. flavus treatment group（P <0.05）,no toxin was detected in each treatment group. L. plantarum destroyed the mold structure,thereby inhibiting its growth,and had the ability to adsorb toxins,which effectively improved the fermentation quality and nutritional components of the whole plant corn silage,and prevented the feed from being infected by molds that leads to reduced safety.

Key words: Lactobacillus plantarum, Aspergillus flavus, mycotoxins, antibacterial substances, whole crop corn silage

ZHAO Ya-ru, XU Qing-fang, GAO Wen-jun, GUO Gang, CHEN Lei, YU Zhu. Study on the Detoxification Characteristics of Antifungal Lactic Acid Bacteria and the Application of Silage[J]. Biotechnology Bulletin, 2021, 37(9): 95-105.

Figures/Tables 16

References 46

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序号 No.	检测项目 Detected item/（μg·kg^-1）	检验结果 Test result
1	黄曲霉毒素B1（AFB1）	26.0
2	黄曲霉毒素B2（AFB2）	2.8
3	黄曲霉毒素G1（AFG1）	未检出（检出限：0.3）
4	黄曲霉毒素G2（AFG2）	未检出（检出限：0.3）
5	黄曲霉毒素M1（AFM1）	未检出（检出限：0.3）

序号 No.	检测项目 Detected item/（μg·kg^-1）	检验结果 Test result
1	黄曲霉毒素B1（AFB1）	26.0
2	黄曲霉毒素B2（AFB2）	2.8
3	黄曲霉毒素G1（AFG1）	未检出（检出限：0.3）
4	黄曲霉毒素G2（AFG2）	未检出（检出限：0.3）
5	黄曲霉毒素M1（AFM1）	未检出（检出限：0.3）

pH	抑菌活性Inhibition of mould activity/mm
CFS（3.5）	+ + +
4.0	+ + +
5.0	+
6.0	-

pH	抑菌活性Inhibition of mould activity/mm
CFS（3.5）	+ + +
4.0	+ + +
5.0	+
6.0	-

天数Days/d	微生物Microorganism	CK	L	A	L+A
0	乳酸菌Lactic acid bacteria	3.24±0.15^b	4.27±0.09^a	3.23±0.13^b	4.27±0.05^a
24		6.45±0.17^d	8.51±0.23^a	7.60±0.06^c	8.47±0.09^b
48		5.23±0.07^b	6.33±0.16^a	5.29±0.06^b	6.30±0.04^a
0	霉菌Mold	1.42±0.42^a	1.43±0.25^a	1.47±0.12^a	1.44±0.06^a
24		0	0	0	0
48		0	0	0	0