Isolation,Identification of Phenol-degrading Pseudoxanthomonas sp. BF-6 and Its Degradation Characteristics and Pathway

doi:10.13560/j.cnki.biotech.bull.1985.2021-0124

Abstract

Abstract:

The aim of this work is to isolate phenol-degrading bacteria and to explore the characteristics and pathways of phenol degradation. Continuous enrichment and dilution plate method were applied to isolate the phenol-degrading bacteria,and single factor test was to study the characteristics of phenol degradation. The metabolic pathway of degrading by the strain phenol was deduced by determining the activities of phenol hydroxylase and catechol dioxygenase. A phenol-degrading strain BF-6,growing with phenol as the sole carbon source,was isolated and named as BF-6. Strain BF-6 was identified as Pseudoxanthomonas sp. by using 16S rRNA sequence analysis and physiological and biochemical tests. Strain BF-6 degraded phenol well in the temperature range of 20-37℃ and pH range of 5.0-10.0;under this condition,strain BF-6 with 5% inoculum degraded 100 mg/L phenol by 97.79% in 36 h and 200 mg/L phenol by 97.58% in 96 h. Cell extracts of strain BF-6 showed enzymatic activity of phenol hydroxylase and catechol 1,2-dioxygenase after induction by phenol,indicating that the strain degraded phenol via ortho-pathway. In conclusion,phenol-degrading bacterium BF-6 is identified as Pseudoxanthomonas sp.,and it degrades phenol under a wide range of temperature and pH conditions. Phenol is degraded via ortho-pathway by strain BF-6. This study provides a new strain for microbial-degration of low-concentration phenol.

Key words: phenol, Pseudoxanthomonas sp., isolation and identification, degradation characterization, degradation pathway

WEI Xiao-bo, HOU Ying, CHENG Hao-jie, QIN Cui-li, NIU Ming-fu, XU Jian-qiang. Isolation,Identification of Phenol-degrading Pseudoxanthomonas sp. BF-6 and Its Degradation Characteristics and Pathway[J]. Biotechnology Bulletin, 2021, 37(10): 72-80.

Figures/Tables 10

References 23

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项目Item	结果Result	项目Item	结果Result
葡萄糖Glucose	+	头孢克肟Cefixime	+
蔗糖Sucrose	+	头孢克洛Cefaclor	+
乳糖Lactose	+	头孢拉定Cefradine	+
木糖Xylose	-	呋喃唑酮Furazolidone	+
甘露醇Mannitol	-	杆菌肽Bacitracin	+
甲醇Methanol	-	阿奇霉素Azithromycin	-
乙醇Ethanol	+	四环素Tetracycline	-
牛肉膏Beef paste	+	卡那霉素Kanamycin	-
蛋白胨Peptone	+	诺氟沙星Norfloxacin	-
酵母粉Yeast powder	+	革兰氏染色Gram stain	-
尿素Urea	+	甲基红试验Methyl red test	-
（NH₄）₂SO₄	+	V-P试验V-P test	-
KNO₃	+	过氧化氢酶试验Catalase test	+
NaNO₂	+	淀粉水解酶试验Starch hydrolase test	-
青霉素Penicillin	+	反硝化试验Denitrification experiment	-
阿莫西林Amoxicillin	+	明胶液化试验Gelatin liquefaction test	-
氨苄西林Ampicillin	+	最适生长温度Optimal growth temperature	37℃
链霉素Streptomycin	+	最适生长pH Optimal growth pH	8.0

项目Item	结果Result	项目Item	结果Result
葡萄糖Glucose	+	头孢克肟Cefixime	+
蔗糖Sucrose	+	头孢克洛Cefaclor	+
乳糖Lactose	+	头孢拉定Cefradine	+
木糖Xylose	-	呋喃唑酮Furazolidone	+
甘露醇Mannitol	-	杆菌肽Bacitracin	+
甲醇Methanol	-	阿奇霉素Azithromycin	-
乙醇Ethanol	+	四环素Tetracycline	-
牛肉膏Beef paste	+	卡那霉素Kanamycin	-
蛋白胨Peptone	+	诺氟沙星Norfloxacin	-
酵母粉Yeast powder	+	革兰氏染色Gram stain	-
尿素Urea	+	甲基红试验Methyl red test	-
（NH₄）₂SO₄	+	V-P试验V-P test	-
KNO₃	+	过氧化氢酶试验Catalase test	+
NaNO₂	+	淀粉水解酶试验Starch hydrolase test	-
青霉素Penicillin	+	反硝化试验Denitrification experiment	-
阿莫西林Amoxicillin	+	明胶液化试验Gelatin liquefaction test	-
氨苄西林Ampicillin	+	最适生长温度Optimal growth temperature	37℃
链霉素Streptomycin	+	最适生长pH Optimal growth pH	8.0