Study on the Reduction Characteristics of Cr（VI）by Two Species of Microorganisms

doi:10.13560/j.cnki.biotech.bull.1985.2021-0136

Abstract

Abstract:

This work aims to study the reduction ability and characteristics of two species of microorganisms to Cr（VI）. The abilities of two species of microorganisms to reduce Cr（VI）and the influence of pH and electron donor on its reduction of Cr（VI）were explored by continuous culturing in lab condition,combining characterization methods the detoxification mechanism of Stenotrophomonas sp. was studied The results showed that the tolerance of Stenotrophomonas sp. and Bacillus mucilaginosus to Cr（VI）was 400 and 200 mg/L. Under neutral conditions,the removal rate of 45.5 and 18.5 mg/L Cr（VI）within 72 h was 100%. Under weakly alkaline conditions,the activities of both microorganisms were strong. At pH 8,removal rates of 50 mg/L Cr（VI）by Stenotrophomonas sp. and B. mucilaginosus within 72 h were 98.1% and 49.2% respectively. Sodium acetate and sodium lactate promoted the removal rate of 50 mg/L Cr（VI）by Stenotrophomonas sp. to be 100% within 60 h. Sodium lactate and glucose increased the removal rate of 50 mg/L Cr（VI）by B. mucilaginosus from 49.2% to 61.9% and 73.2% within 72 h,respectively. The analysis of the reduced products showed that Stenotrophomonas sp. adsorbed Cr（VI）on the surface of the body and efficiently reduced Cr（VI）to Cr（III）. Stenotrophomonas sp. and B. mucilaginosus can be used as high quality strain for remediation of heavy metal Cr（VI）pollution.

Key words: heavy metal pollution, microorganism, Cr（VI）, adsorption, reduction

HUANG Yu-xi, CHENG Shun-li, HE Ling-ling, XIAO Jin-bin, REN Qiu-he, PENG Zi-han, ZHOU Zhen, FANG Yu-mei. Study on the Reduction Characteristics of Cr（VI）by Two Species of Microorganisms[J]. Biotechnology Bulletin, 2021, 37(10): 63-71.

Figures/Tables 8

Fig.1 Effect of different Cr（VI）concentration on the growth of microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

Fig.2 Maximum reduction of Cr（VI）by two species of microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

Fig.3 Residual amount of Cr（VI）in 72 h

Fig.4 Maximum Cr（VI）concentration that can be completely degraded

Fig.5 Effect of initial pH on the reduction of Cr（VI）by microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

Fig.6 Effect of different electron donors on the reduction of Cr（VI）by microorganisms A:Stenotrophomonas sp.;B:Bacillus mucilaginosus

Fig.7 Scanning electron microscopy before and after reduction of Cr（VI）by Stenotrophomonas sp. A:Before reduction. B:After reduction

Fig.8 Full range XPS spectra and spectra of Cr2p for the reduction products of Stenotrophomonas sp. A:Full range XPS spectra. B:Spectra of Cr2p

References 31

[1]	Lyu H, Zhao H, Tang J, et al. Immobilization of hexavalent chromium in contaminated soils using biochar supported nanoscale iron sulfide composite[J]. Chemosphere, 2018, 194:360-369. doi: 10.1016/j.chemosphere.2017.11.182 URL
[2]	何力为, 李彬, 宁平, 等. 利用一氧化碳工业废气解毒铬渣的方法及条件优化[J]. 环境工程学报, 2018, 12(9):2617-2626.
	He LW, Li B, Ning P, et al. Method and process optimization of applying CO waste gas to detoxify chromite ore processing residue[J]. Chin J Environ Eng, 2018, 12(9):2617-2626.
[3]	崔永高. 铬污染土壤和地下水的修复技术研究进展[J]. 工程地质学报, 2017, 25(4):1001-1009.
	Cui YG. Research progress on remediation technology of chromium contaminated soils and groundwater in Shanghai[J]. J Eng Geol, 2017, 25(4):1001-1009.
[4]	张宏陶. 生活饮用水标准;检验法方法注解[M]. 重庆: 重庆大学出版社, 1993.
	Zhang HT. Notes to the Drinking Water Standard TestMethods[M]. Chongqing: Chongqing University Press, 1995.
[5]	张双庆. 铬价态分析方法的研究进展[J]. 卫生研究, 2019, 48(6):1037-1040.
	Zhang SQ. Research progress of chromium valence analysis methods[J]. J Hyg Res, 2019, 48(6):1037-1040.
[6]	Rai D, Sass BM, Moore DA. Chromium(III)hydrolysis constants and solubility of chromium(III)hydroxide[J]. Inorg Chem, 1987, 26(3):345-349. doi: 10.1021/ic00250a002 URL
[7]	Selvi K. Removal of Cr(VI)from aqueous solution by adsorption onto activated carbon[J]. Bioresour Technol, 2001, 80(1):87-89. doi: 10.1016/S0960-8524(01)00068-2 URL
[8]	Cavaco SA, Fernandes S, Quina MM, et al. Removal of chromium from electroplating industry effluents by ion exchange resins[J]. J Hazard Mater, 2007, 144(3):634-638. pmid: 17336455
[9]	刘芳. 还原沉淀法对含铬重金属废水的处理研究[J]. 环境污染与防治, 2014, 36(4):54-59.
	Liu F. Treatment of chromium containing heavy metal wastewater by reduction and sedimentation process[J]. Environ Pollut Control, 2014, 36(4):54-59.
[10]	Frenzel I, Holdik H, Barmashenko V, et al. Electrochemical reduction of dilute chromate solutions on carbon felt electrodes[J]. J Appl Electrochem, 2006, 36(3):323-332. doi: 10.1007/s10800-005-9074-y URL
[11]	虞少嵚, 熊道文, 陈湘斌, 等. 周期换向电絮凝法用于处理含铬废水研究[J]. 中国环境科学, 2014, 34(1):118-122.
	Yu SQ, Xiong DW, Chen XB, et al. Treatment of wastewater containing chromium with periodic reversal electrocoagulation[J]. China Environ Sci, 2014, 34(1):118-122.
[12]	肖文丹, 叶雪珠, 孙彩霞, 等. 铬耐性菌对土壤中六价铬的还原作用[J]. 中国环境科学, 2017, 37(3):1120-1129.
	Xiao WD, Ye XZ, Sun CX, et al. The effect of chromium-resistant bacteria on reduction of hexavalent chromium in soils[J]. China Environ Sci, 2017, 37(3):1120-1129.
[13]	Romanenko VI, Koren’kov VN. Pure culture of bacteria using chromates and bichromates as hydrogen acceptors during development under anaerobic conditions[J]. Mikrobiologiia, 1977, 46(3):414-417. pmid: 895551
[14]	肖伟, 王磊, 李倬锴, 等. 六价铬还原细菌Bacillus cereus S5. 4还原机理及酶学性质研究[J]. 环境科学, 2008, 29(3):751-755. pmid: 18649539
	Xiao W, Wang L, Li ZK, et al. Mechanisms and enzymatic characters of hexavalent chromium reduction by Bacillus cereus S5. 4[J]. Environ Sci, 2008, 29(3):751-755. pmid: 18649539
[15]	Zaman Tanu F. Bacterial tolerance and reduction of chromium(VI)by Bacillus cereus isolate PGBw4[J]. Am J Environ Prot, 2016, 5(2):35.
[16]	Liu H, Guo L, Liao S, et al. Reutilization of immobilized fungus Rhizopus sp. LG04 to reduce toxic chromate[J]. J Appl Microbiol, 2012, 112(4):651-659. doi: 10.1111/j.1365-2672.2012.05257.x pmid: 22332919
[17]	Sivakumar D. Biosorption of hexavalent chromium in a tannery industry wastewater using fungi species[J]. Global Journal of Environmental Science and Management, 2016, 2(2):105-124.
[18]	杨重, 徐天怡, 刘奕含, 等. 耐盐菌Staphylococcus sp. YZ-1和Bacillus cereus CC-1的Cr(Ⅵ)脱毒特性与机理[J]. 微生物学通报, 2020, 47(1):66-75.
	Yang Z, Xu T Y, Liu Y H, et al. Cr(VI)detoxification characteristics of salt-tolerant Staphylococcus sp. YZ-1 and Bacillus cereus CC-1[J]. Microbiol China, 2020, 47(1):66-75.
[19]	朱文杰. Leucobacter sp. CRB1菌还原铬(VI)的机理及其在铬渣解毒中的应用[D]. 长沙:中南大学, 2008.
	Zhu WJ. Mechanism of Cr(VI)reduction with Leucobacter sp. CRB1 and its application in detoxification of chromite ore processing residue[D]. Changsha:Central South University, 2008.
[20]	陈国才, 梁好, 刘传胜, 等. 不同电子供体和受体对零价铁与微生物协同还原六价铬的影响[J]. 环境工程学报, 2017, 11(6):3487-3492.
	Chen GC, Liang H, Liu CS, et al. Effect of electron donors and acceptors on Cr(Ⅵ)reduction by combination of zero valent iron and microorganisms[J]. Chin J Environ Eng, 2017, 11(6):3487-3492.
[21]	崔跃琳, 施春红, 张宝刚, 等. 微生物还原钒、铬过程的电子供体研究进展[J]. 环境科技, 2019, 32(3):73-78.
	Cui YL, Shi CH, Zhang BG, et al. Research progress in electron donors for microbial reduction of vanadium and chromium[J]. Environ Sci Technol, 2019, 32(3):73-78.
[22]	杜艳影, 刘小红, 李劲, 等. Shewanella oneidensis MR-1对Cr(Ⅵ)的还原及其影响因素[J]. 中国环境科学, 2018, 38(7):2740-2745.
	Du YY, Liu XH, Li J, et al. Reduction of Cr(Ⅵ)by Shewanella oneidensis MR-1 and its influencing factors[J]. China Environ Sci, 2018, 38(7):2740-2745.
[23]	Yu ZH, Zhang XD, Huang YM. Magnetic chitosan-iron(III)hydrogel as a fast and reusable adsorbent for chromium(VI)removal[J]. Ind Eng Chem Res, 2013, 52(34):11956-11966. doi: 10.1021/ie400781n URL
[24]	汤洁, 王卓行, 徐新华. 铁屑-微生物协同还原去除水体中Cr(Ⅵ)研究[J]. 环境科学, 2013, 34(7):2650-2657. pmid: 24027995
	Tang J, Wang ZX, Xu XH. Removal of Cr(Ⅵ)by iron filings with microorganisms to recover iron reactivity[J]. Environ Sci, 2013, 34(7):2650-2657. pmid: 24027995
[25]	Pal A, Paul AK. Aerobic chromate reduction by chromium-resistant bacteria isolated from serpentine soil[J]. Microbiol Res, 2004, 159(4):347-354. doi: 10.1016/j.micres.2004.08.001 URL
[26]	刘奎艳, 祝天宇, 范鑫, 等. 一株耐Cr(Ⅵ)木霉的筛选鉴定及其Cr(Ⅵ)还原特性[J]. 黑龙江大学自然科学学报, 2018, 35(3):324-333.
	Liu KY, Zhu TY, Fan X, et al. Study on the characteristics of Cr(VI)removal by Cr(VI)-resistant fungi[J]. J Nat Sci Heilongjiang Univ, 2018, 35(3):324-333.
[27]	徐天生, 欧杰, 马晨晨. 微生物还原Cr(Ⅵ)的机理研究进展[J]. 环境工程, 2015, 33(1):32-36.
	Xu TS, Ou J, Ma CC. Advances in mechanism about microbial reduction of Cr(ⅵ)[J]. Environ Eng, 2015, 33(1):32-36.
[28]	郝孔利, 张子辉, 王珊珊, 等. 季也蒙毕赤酵母菌还原Cr(Ⅵ)的特性研究[J]. 华中农业大学学报, 2020, 39(2):63-70.
	Hao KL, Zhang ZH, Wang SS, et al. Reduction characteristics of Cr(Ⅵ)by Pichia guilliermondii[J]. J Huazhong Agric Univ, 2020, 39(2):63-70.
[29]	朱文杰, 龙怀中, 杨志辉, 等. Leucobacter对Cr(Ⅵ)的还原及其还原产物的成分分析[J]. 中南大学学报:自然科学版, 2008, 39(3):443-447.
	Zhu WJ, Long HZ, Yang ZH, et al. Bio-reduction of Cr(Ⅵ)by Leucobacter and element analysis of reduction products[J]. J Central South Univ:Sci Technol, 2008, 39(3):443-447.
[30]	Aravindhan R, Fathima A, Selvamurugan M, et al. Adsorption, desorption, and kinetic study on Cr(III)removal from aqueous solution using Bacillus subtilis biomass[J]. Clean Technol Environ Policy, 2012, 14(4):727-735. doi: 10.1007/s10098-011-0440-7 URL
[31]	刘鹏宇, 王晓琴, 常青, 等. 铝炭微电解去除废水中六价铬的可行性研究[J]. 中国环境科学, 2019, 39(10):4164-4172.
	Liu PY, Wang XQ, Chang Q, et al. Feasibility study on the removal of chromium(Ⅵ)containing from heavy metal wastewater by aluminum-carbon micro electrolysis[J]. China Environ Sci, 2019, 39(10):4164-4172.