Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (2): 67-74.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0757

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Composition Features of Microbial Community in the Rhizospheric Soil of Bletilla striata with Root Rot

ZHAO Lin-yan1(), GUAN Hui-lin1, XIANG Ping2, LI Ze-cheng3, BAI Yu-long3, SONG Hong-chuan1, SUN Shi-zhong1, XU Wu-mei1()   

  1. 1. Yunnan Provincial Observation and Research Station of Soil Degradation and Restoration for Cultivating Plateau Traditional Chinese Medicinal Plants,Yunnan Normal University,Kunming 650500
    2. Institute of Environmental Remediation and Human Health,Southwest Forestry University,Kunming 650224
    3. Kunming Yingwu Agricultural Technology Co.,Ltd.,Kunming 650500
  • Received:2021-06-13 Online:2022-02-26 Published:2022-03-09
  • Contact: XU Wu-mei E-mail:zhaolinyan1166@163.com;xuwumei@ynnu.edu.cn

Abstract:

Analyzing the composition features of microbial community in the rhizospheric soil of Bletilla striata with root rot would provide the scientific basis for understanding the microbial mechanisms leading to the root rot of B. striata and taking corresponding prevention and control measures. The Illumina HiSeq high-throughput sequencing was used to compare and analyze the microbial community structure in the rhizospheric soils of root-rot and healthy B. striata. Also,the soil physicochemical properties and enzyme activities were quantitatively analyzed. The dominant fungi were Ascomycota and Mortierellomycota,and the dominant bacteria were Proteobacteria and Bacteroidetes in the rhizospheric soil of B. striata. Compared with the healthy individuals,the relative abundance of Ascomycota significantly increased,while the relative abundance of Mortierellomycota decreased. The relative abundance of pathogenic Fusarium and Erysiphe significantly increased in the rhizospheric soil of root-rot B. striata(P<0.05). Multivariate statistical analyses showed the significant differentiation of bacterial and fungal community in the rhizospheric soils between healthy and root-rot B. striata. In addition,the activities of urease,protease and sucrase,and available potassium content were higher in the rhizospheric soil of healthy individuals;while soil pH and the contents of organic matter,NH4+-N,and NO3--N were lower. The root-rot disease of B. striata could be related to the increased relative abundance of pathogenic microorganisms,the changes of microbial community structure,the decreased soil enzyme activity,and the imbalance of plant nutrients.

Key words: Bletilla striata, root-rot, soil microbial community, high-throughput sequencing