Bacterial Communities in a Middle School Campus Assessed by High-throughput Sequencing

doi:10.13560/j.cnki.biotech.bull.1985.2020-0134

Abstract

Abstract: The elementary and middle school campus is a special living environment with the characteristics of large population density,active microbial transmission and communication. The microbial community structure and transmission in campus is a key factor impacting the microbial formation and development of adolescents’ healthy. Taking a middle school campus as the research object,the microbial community structure in different places on the campus was explored,and an attempt was made to clarify the campus microbial community and its characteristic distribution. Microbial samples were collected from where human body may easily be in touch to,such as the armrests in the classrooms and gym stadium,the doors and handrails in laboratories,and handrails in canteen. The V4 regions of bacterial 16S rRNA genes for the samples were sequenced at high-throughput using Illumina HiSeq platform. The results showed that buildings of different usages exhibited distinct microbial communities. There were significant differences in their relative abundances though the microorganisms on the surface of the objects in each building mainly belonged to Proteobacteria,Firmicutes and Actinobacteria at the phylum level and Enhydrobacter,Streptococcus and Staphylococcus at the genus level. For example,Acinetobacter and Aerococcus in the canteen samples showed higher abundances but were absent in buildings at the teaching area. The microbial diversity on the armrest surface of the gym stairs was significantly higher than that of other facilities. Through a preliminary analysis of the composition of bacteria in different locations on the campus,we found that the microbial community in the different buildings of the school campus was determined by their usages,and speculated that the distribution of these characteristics should be closely related to different types of teaching and life activities. This study provides a reference for further studying the impact of the campus environment on adolescents’ physical health.

Key words: campus, community structure, high-throughput sequencing, microbial diversity

HUANG Ting, FANG Yuan, FENG Zhou, SHEN He, NIE Yong, ZHENG Xin, WANG Jia-quan, XU Zi-mu. Bacterial Communities in a Middle School Campus Assessed by High-throughput Sequencing[J]. Biotechnology Bulletin, 2020, 36(8): 96-103.

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