Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (5): 93-99.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0937

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Cloning and Bioinformatics Analysis of Blue-light Receptor EaWC 1 Gene in Elsinoë arachidis

LI Yang(), ZHANG Xiao-tian, PIAO Jing-zi, ZHOU Ru-jun(), LI Zi-bo, GUAN Hai-wen   

  1. College of Plant Protection,Shenyang Agricultural University,Shenyang 110866
  • Received:2021-07-22 Online:2022-05-26 Published:2022-06-10
  • Contact: ZHOU Ru-jun E-mail:liyang20210713@163.com;zhourujun@syau.edu.cn

Abstract:

Light is an important information carrier for fungi to perceive and adapt to the environment factors,regulate a variety of physiological and biochemical processes. Elsinochromes(ESC)is an important virulence factor of Elsinoë arachidis causing peanut scab. In order to further reveal mechanism of light regulation on ESC production,gene cloning,bioinformatics analysis and expression of the blue-light receptor gene EaWC 1 were conducted. The results showed that the blue light receptor gene EaWC-1 had a complete reading frame(ORF)with 3 261 bp in length that encoded a protein of 1 086 amino acids. The molecular weight of the protein was 11.95 kD,the theoretical isoelectric point was 8.81. Subcellular localization analysis showed that EaWC-1 was localized in nucleus and was a hydrophilic protein. qPCR analysis indicated that the expression of EaWC 1 gene was positively regulated by blue light,and its expression pattern was significantly and positively correlated with ESC toxin accumulation. This result would provide a theoretical foundation for elucidating the biological functions of blue light receptors of E. arachidis and revealing the photo-regulation mechanism and regulatory network of ESC toxin biosynthesis.

Key words: Elsinoë arachidis, blue-light receptor, gene cloning, bioinformatics analysis, expression analysis