Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (9): 106-115.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0073

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Identification and Drug Resistance of Escherichia coli Simultaneously Producing Carbapenemase NDM-1 and NDM-5

LI Hai-li(), LANG Li-min, ZHANG Qing-xian, YOU Yi, ZHU Wen-hao, WANG Zhi-fang, ZHANG Li-xian, WANG Ke-ling   

  1. Institute of Animal Science and Veterinary Medicine,Henan Academy of Agricultural Sciences,Zhengzhou 450002
  • Received:2022-01-16 Online:2022-09-26 Published:2022-10-11

Abstract:

In order to understand the prevalence of carbapenemase-carrying NDM-1 and its subtypes in pig farms,87 strains of Escherichia coli were isolated and identified from piglet diarrhea cases. Carbapenemase-resistant genes NDM-1 and NDM-5 were amplified and sequenced. An E. coli strain carrying both NDM-1 and NDM-5 was identified from 87 strains of E. coli and named HN2106. In order to further understand the whole genome of HN2106 and the plasmids and drug-resistant genes carried by HN2106,16S rRNA strain identification of NH2106 was conducted,blaNDM drug-resistant gene was confirmed,and whole genome sequencing was performed. BLAST and MEGA software were used for bioinformatics analysis and phylogenetic tree analysis. The results showed that the strain contained 7 plasmids(plasmid A,plasmid B,plasmid C,plasmid D,plasmid E,plasmid F,and plasmid G),in 3 types,were as Col,IncFII,and IncQ1. The strain simultaneously carried gene NDM-1 and NDM-5,they belonged to a β -lactamase of group B,in which blaNDM-1 was located at plasmid pNDM5-HN2106(plasmid C),blaNDM-5 was located at plasmid pNDM5-HN2106(plasmid B). They mediated blaNDM-1 and blaNDM-5 resistance genes,respectively,and were important vectors for the transmission of drug-resistant bacteria. The prediction of whole genome resistance gene demonstrated that HN2106 also carried 21 types of antibiotic resistance genes. The resistances of HN2106 strain to 85 antibiotics were tested,and the results showed that HN2106 was resistant to imipenem,penicillin G,et al.,as well as it was sensitive to sulfamethoxazole,cefmetazole,et al. The above results reveal that NDM-1 drug-resistant plasmid is an important vector of NDM-1 and its subtype resistance genes,which may bring great hidden dangers and threats to healthy breeding and public health safety in the breeding industry. In order to provide theoretical reference for the prevention and treatment of clinical drug-resistant bacterial infection,it is necessary to strengthen the rational application of antibacterial drugs and the prevention and control measures of infection,and pay more attentions to the monitoring of bacterial resistances.

Key words: carbapenase, NDM-1, NDM-5, Escherichia coli, bacterial drug resistance