Antitumor Activity of the Ethanol Extract of Pleurotus ferulae and the Extraction Procedure of Triterpenoid Components

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.030

Abstract

Abstract: This work aims to explore the antitumor activity of alcohol soluble components from Pleurotus ferulae（PFECs）,and to optimize the extraction condition of triterpenoids from P. ferulae（PFTPs）for maximum extraction yield. MTT was used to detect the inhibitory activity of PFECs to 5 kinds of tumor cells,no significant inhibition to 2 kinds of normal cells was observed. Ultrasonic-assisted,hot-water leaching with ultrasonic-assisted,single-factor experiment,and response surface methodology were jointly employed to optimize the extraction process of PFTPS. The results showed that PFECs presented significant antitumor activity. The optimal process parameters for the PFTPs extraction were：the ratio of ethanol to material 1∶19（g/mL）,extraction time 22 min and extraction temperature 7℃,and the extracting yield of PFTPs was 4.42%.

Key words: Pleurotus ferulae, ethanol extracts, active components, antitumor activity, triterpenoids, optimization of extraction, response surface methodology

WANG Wei-lan CHEN Kai-xu LIU Jun DUAN Wei-wei XU Ya-nan ZHANG Fu-chun. Antitumor Activity of the Ethanol Extract of Pleurotus ferulae and the Extraction Procedure of Triterpenoid Components[J]. Biotechnology Bulletin, 2016, 32(7): 206-216.

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