Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (1): 187-198.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1033

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Identification of Zinc Finger Protein DnaJ-Like Gene Family in Capsicum annuum and Its Expression Analysis Responses to High Temperature Stress

DUAN Min-jie1(), LI Yi-fei1, YANG Xiao-miao1, WANG Chun-ping2, HUANG Qi-zhong1, HUANG Ren-zhong1, ZHANG Shi-cai1()   

  1. 1. Institute of Vegetables and Flowers, Chongqing Academy of Agricultural Sciences, Chongqing 401329
    2. Institute of Biotechnology, Chongqing Academy of Agricultural Sciences, Chongqing 401329
  • Received:2022-08-23 Online:2023-01-26 Published:2023-02-02
  • Contact: ZHANG Shi-cai E-mail:swemart@163.com;shicaiz@126.com

Abstract:

Plant DanJ-Like zine finger protein(DNAJE)is one of the important protein found in recent years, which plays an important role in photosynthesis, development and motility of plastids and the responses of plants to stresses. Here, the DNAJE whole gene family(CaDNAJE)was identified by the whole genome data of pepper(Capsicum annuum, and the characteristic of gene and protein, comparative evolution, collinearity and transcriptional expression under high temperature stress were analyzed. Totally, 28 members of CaDNAJE genes were identified, and they were distributed unevenly on 10 chromosomes and 3 Scaffolds. The amino acid length, molecular mass and isoelectric point were between 99-470 aa, 10.05-52.26 kD and 4.75-9.64, respectively. Moreover, these encoded proteins were mainly located in the chloroplast and nucleus. The comparative evolution of pepper, tomato(Solanum lycopersicum)and Arabidopsis analysis can be divided into 2 subgroups: GroupⅠ and GroupⅡ, and the CaDNAJE protein in the same subgroup had similar conserved motifs and gene structures. Cis-acting element analysis in the promoter region showed that CaDNAJEs contained a large numbers of functional elements related to light response, hormone response and stress. There were 10 pairs of collinearity genes between pepper and Arabidopsis, but only one pair within pepper species. The transcriptome analysis of different pepper tissues indicated that most members of GroupⅠ were highly expressed in all tissues, while most members of GroupⅡ had low or no expression except for CaDNAJE2 and CaDNAJE9. The content of hydrogen peroxide in pepper leaves increased rapidly and then decreased. The content of malondialdehyde kept increasing and peroxidase activity also had been improved to different extent. High temperature stress induced the high expression of the heat shock factor/heat shock protein(Hsf/HSP)and CaDNAJE genes, which played a regulation role in different stress time. It is suggested that CaDNAJE family genes are involved in response to high temperature stress and then increased tolerance to reduce cell damage.

Key words: pepper, DnaJ-Like zinc finer protein, genome-wide identification, high temperature stress, gene expression, regulation