Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (7): 288-297.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1430
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ZHOU Zhen-chao1(), ZHENG Ji2, SHUAI Xin-yi1, LIN Ze-jun1, CHEN Hong1()
Received:
2022-11-21
Online:
2023-07-26
Published:
2023-08-17
Contact:
CHEN Hong
E-mail:zhouzc@zju.edu.cn;chen_hong@zju.edu.cn
ZHOU Zhen-chao, ZHENG Ji, SHUAI Xin-yi, LIN Ze-jun, CHEN Hong. High-throughput Profiling and Analysis of Shared Antibiotic Resistance Genes in Human Feces, Skin and Water Environments[J]. Biotechnology Bulletin, 2023, 39(7): 288-297.
Fig. 1 Relative abundances of ARGs in humans and water samples Red points indicate commonly shared ARGs in human feces, skin and water(n =70). Error bars indicate min/max values, and centre bars indicate median
Fig. 4 Taxa discriminates among human feces, skin and water microbiota as determined by LEfSe A: Kingdom- to family-level taxa were shown in the phylogenetic tree. Colored taxa are discriminative between categories(alpha value of < 0.05 for both the Kruskal-Wallis and pairwise Wilcoxon rank-sum tests, and LDA effect size of ≥ 4.0), circle size is proportional to the taxa abundance. B, C: Relative abundances of discriminative microbial phyla(B)and families(C)in human feces, skin and water microbiota. Only taxa with a mean relative abundance of ≥ 1% in one category are shown. Error bars indicate standard deviation(s. d.), centre bars indicate median, and black points indicate outliers
Fig. 6 Network analysis of all detected ARGs, MGEs and bacteria(A)and shared ARGs, MGEs and bacteria(B) The nodes were colored according to modularity class. A connection represents a strong(Spearman's correlation coefficient(ρ)>0.7)and significant(P-value<0.01)correlation. Node size weighted according to the number of connections(that is the degree)and edges weighted according to the correlation coefficient
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