Abstract:

Streptomyces sp. FXP04 is a gram-positive actinomycete isolated from the rhizosphere soil of healthy potato in Keshan county, Qiqihar, which has obvious antagonistic effect against Phytophthora infestans. This work aims to analyze the whole genome sequence information of strain FXP04, to explore the mechanism of action as well as the gene resources for the biosynthesis of secondary metabolites. The whole genome of Streptomycessp. FXP04 was sequenced by Illumina and PacBio platform, and gene prediction, functional annotation, comparative genomics analysis and synthesis gene cluster prediction of secondary metabolite were carried out. The genomic size of strain FXP04 was 4 535 201bp, encoding a total of 5 037 genes, with a GC content of 72.95%, including three 5S rRNA, three 16S rRNA, three 23S rRNA, 33 tRNA and 23 sRNA. It contained 1 699 tandem repeats，1 284 microsatellite DNA,188 microsatellite DNA. It was annotated to 2 088, 2 319, 1 530, 73, 139 and 14 genes in GO, COG, KEGG, CAZy, VFDB and ARDB databases, respectively. At the same time, it was predicted that there were 10 secondary metabolite synthesis gene clusters in strain FXP04, which encoded bacteriostatic substances such as piericidin, youssoufene and so on. The internal mechanism of strain FXP04 was analyzed by genome sequencing, which provides further theoretical basis for the development and utilization of strain FXP04.

Key words: Streptomyces, genome, gene annotation