Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (11): 340-349.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0624
Previous Articles Next Articles
LIU Yuan-yuan1(), WEI Chuan-zheng1, XIE Yong-bo1, TONG Zong-jun2, HAN Xing2, GAN Bing-cheng2, XIE Bao-gui1(), YAN Jun-jie2()
Received:
2023-06-30
Online:
2023-11-26
Published:
2023-12-20
Contact:
XIE Bao-gui, YAN Jun-jie
E-mail:lyylyy0815@163.com;mrcfafu@163.com;yanjunjie@caas.cn
LIU Yuan-yuan, WEI Chuan-zheng, XIE Yong-bo, TONG Zong-jun, HAN Xing, GAN Bing-cheng, XIE Bao-gui, YAN Jun-jie. Characteristics of Class II Peroxidase Gene Expression During Fruiting Body Development and Stress Response in Flammulina filiformis[J]. Biotechnology Bulletin, 2023, 39(11): 340-349.
引物名称Primer name | 引物序列Primer sequence(5'-3') |
---|---|
qFfGAPDH-F qFfGAPDH-R | CCTCTGCTCACTTGAAGGGT |
GCGTTGGAGATGACTTTGAA | |
qFfCIIp1-F qFfCIIp1-R | TGACTTTCGCATTCCCCGAA |
ACAGAGAGTCAAGGCAACGG | |
qFfCIIp2-F | AAGTCGAAAGGCGGGACAAT |
qFfCIIp2-R | CCGTCAGGATTGATGGTCGT |
Table 1 Sequences of primers
引物名称Primer name | 引物序列Primer sequence(5'-3') |
---|---|
qFfGAPDH-F qFfGAPDH-R | CCTCTGCTCACTTGAAGGGT |
GCGTTGGAGATGACTTTGAA | |
qFfCIIp1-F qFfCIIp1-R | TGACTTTCGCATTCCCCGAA |
ACAGAGAGTCAAGGCAACGG | |
qFfCIIp2-F | AAGTCGAAAGGCGGGACAAT |
qFfCIIp2-R | CCGTCAGGATTGATGGTCGT |
Fig. 3 Phylogenetic tree of F. filiformis FfCIIp and CIIp from other basidiomycetes The bar: Evolutionary distance. The values above branches: The degree of bootstrap support from a 1000 replicate analysis. The taxon names of branches: composed of ID in database, Latin name of species and sequence name. Square: F. filiformis FfCIIp sequence
Fig. 4 Multiple sequence alignment and conserved domain analysis of CIIp amino acid sequences from F. filiformis and other fungi Base background color: The darker the color, the more conservative the site. The transverse line: Peroxidase domain. FfCIIp1, FfCIIp2: Class II peroxidase sequences of F. filiformis. Black solid inverted triangle: Three acidic amino acid residues in the MnP and VP families are crucial for Mn2+ oxidation. Hollow inverted triangle: Tryptophan residue in the LiP and VP families is responsible for oxidation of phenolic compounds. GP: No above 4 catalytic sites
Fig. 5 Expression patterns of FfCIIp during fruiting body tissues of F. filiformis A: Differential expressions of FfCIIp1 in fruiting body samples(PR: Primordium stage. DI: Differentiation stage. EL-stipe: The stipe of elongation stage. EL-pileus: The pileus of elongation stage. MA-stipe: The stipe of maturation stage. MA-pileus: The pileus of maturation stage). B: Differential expression of FfCIIp2 in fruiting body samples. C: Differential expression of FfCIIp1 in different segment of stipe(elo: Fastest elongating region. sta: Stable region, this region no longer elongated). D: Differential expression of FfCIIp2 in different segment of stipe. The different letters over the columns within the same graph denote significant differences. P<0.05, n=3
Fig. 6 Expression pattern of FfCIIp gene after mechanical injury to F. filiformis hyphae A: Fv01 mycelial injury stress induction. B: The expression pattern of FfCIIp1 in response to mechanical injury of F. filiformis. C: The expression pattern of F. filiformis FfCIIp2 in response to mechanical injury of F. filiformis(The ‘*’ over the columns denotes significant differences with 0 h. *: P<0.05, **: P<0.01, ***: P<0.001, n=3)
Fig. 7 Expression pattern of FfCIIp gene after H2O2 stress of F. filiformis hyphae A: The expression pattern of FfCIIp1 in response to H2O2 stress. B: The expression pattern of FfCIIp2 in response to H2O2 stress.(The ‘*’ over the columns denotes significant differences with 0 mmol/L. *: P<0.05,**: P<0.01,***: P<0.001,n=3)
[1] | 彭洋洋, 何焕清, 江涛, 等. 金针菇工厂化瓶装栽培工艺流程与管理要点[J]. 特种经济动植物, 2019, 22(12): 29-32. |
Peng YY, He HQ, Jiang T, et al. Technological process and management points of industrialized bottle cultivation of Flammulina velutipes[J]. Spec Econ Anim Plant, 2019, 22(12): 29-32. | |
[2] |
Dong Z, Xiao YQ, Wu H. Selenium accumulation, speciation, and its effect on nutritive value of Flammulina velutipes(Golden needle mushroom)[J]. Food Chem, 2021, 350: 128667.
doi: 10.1016/j.foodchem.2020.128667 URL |
[3] | 王翠翠, 崔成伟, 陈屏, 等. 金针菇化学成分及药理活性研究进展[J]. 菌物研究, 2021, 19(3): 207-216. |
Wang CC, Cui CW, Chen P, et al. Research advances on chemical constituents and pharmacological activities of Flammulina filiformis[J]. J Fungal Res, 2021, 19(3): 207-216. | |
[4] |
Dowom SA, Rezaeian S, Pourianfar HR. Agronomic and environmental factors affecting cultivation of the winter mushroom or Enokitake: achievements and prospects[J]. Appl Microbiol Biotechnol, 2019, 103(6): 2469-2481.
doi: 10.1007/s00253-019-09652-y pmid: 30685812 |
[5] |
Sakamoto Y. Influences of environmental factors on fruiting body induction, development and maturation in mushroom-forming fungi[J]. Fungal Biol Rev, 2018, 32(4): 236-248.
doi: 10.1016/j.fbr.2018.02.003 URL |
[6] |
Segal LM, Wilson RA. Reactive oxygen species metabolism and plant-fungal interactions[J]. Fungal Genet Biol, 2018, 110: 1-9.
doi: S1087-1845(17)30179-2 pmid: 29225185 |
[7] |
Inupakutika MA, Sengupta S, Devireddy AR, et al. The evolution of reactive oxygen species metabolism[J]. J Exp Bot, 2016, 67(21): 5933-5943.
pmid: 27742750 |
[8] |
Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress[J]. Curr Biol, 2014, 24(10): R453-R462.
doi: 10.1016/j.cub.2014.03.034 URL |
[9] | 崔世瑞, 冯志勇, 陈明杰, 等. 食用菌体内抗氧化酶活性影响因素的研究进展[J]. 微生物学杂志, 2015, 35(4): 87-92. |
Cui SR, Feng ZY, Chen MJ, et al. Advance in effective factors of antioxidant enzyme activity in edible fungi[J]. J Microbiol, 2015, 35(4): 87-92. | |
[10] | 牛红军, 岳鹍, 滕文华, 等. 过氧化物酶和PeroxiBase过氧化物酶数据库[J]. 生命科学研究, 2012, 16(6): 539-544. |
Niu HJ, Yue K, Teng WH, et al. Peroxidases and the database of peroxidases: PeroxiBase[J]. Life Sci Res, 2012, 16(6): 539-544. | |
[11] |
Passardi F, Bakalovic N, Teixeira FK, et al. Prokaryotic origins of the non-animal peroxidase superfamily and organelle-mediated transmission to eukaryotes[J]. Genomics, 2007, 89(5): 567-579.
pmid: 17355904 |
[12] | 王瑞清, 严俊杰, 李依宁, 等. 金针菇细胞色素c过氧化物酶基因(ffccp)及其在菌柄伸长的差异表达初步分析[J]. 菌物学报, 2020, 39(6): 993-1005. |
Wang RQ, Yan JJ, Li YN, et al. Cytochrome c peroxidase gene(ffccp)and its differential expression during stipe elongation in Flammulina filiformis[J]. Mycosystema, 2020, 39(6): 993-1005. | |
[13] |
Kellner H, Luis P, Pecyna MJ, et al. Widespread occurrence of expressed fungal secretory peroxidases in forest soils[J]. PLoS One, 2014, 9(4): e95557.
doi: 10.1371/journal.pone.0095557 URL |
[14] |
Zhang JJ, Hao HB, Wu XL, et al. The functions of glutathione peroxidase in ROS homeostasis and fruiting body development in Hypsizygus marmoreus[J]. Appl Microbiol Biotechnol, 2020, 104(24): 10555-10570.
doi: 10.1007/s00253-020-10981-6 |
[15] |
Du F, Mai Mai Ti NEZYYL, Hu QX, et al. A comparative transcriptome analysis reveals physiological maturation properties of mycelia in Pleurotus tuoliensis[J]. Genes, 2019, 10(9): 703.
doi: 10.3390/genes10090703 URL |
[16] |
Sheng L, Sun XY, Mo CY, et al. Relationship between antioxidant enzymes and sclerotial formation of Pleurotus tuber-regium under abiotic stress[J]. Appl Microbiol Biotechnol, 2023, 107(4): 1391-1404.
doi: 10.1007/s00253-022-12358-3 |
[17] |
Wang Y, Li GQ, Jiao XY, et al. Correction: molecular characterization and overexpression of mnp6 and vp3 from Pleurotus ostreatus revealed their involvement in biodegradation of cotton stalk lignin[J]. Biol Open, 2023, 12(1): bio059735.
doi: 10.1242/bio.059735 URL |
[18] |
Wang Y, Wang XT, Lan WQ, et al. Impacts and tolerance responses of Coprinus comatus and Pleurotus cornucopiae on cadmium contaminated soil[J]. Ecotoxicol Environ Saf, 2021, 211: 111929.
doi: 10.1016/j.ecoenv.2021.111929 URL |
[19] |
Yan JJ, Zhang L, Wang RQ, et al. The sequence characteristics and expression models reveal superoxide dismutase involved in cold response and fruiting body development in Volvariella volvacea[J]. Int J Mol Sci, 2016, 17(1): 34.
doi: 10.3390/ijms17010034 URL |
[20] | 张磊, 仝宗军, 严俊杰, 等. 金针菇小G蛋白Ran的序列特征与表达分析[J]. 食用菌学报, 2018, 25(1): 13-19, 127. |
Zhang L, Tong ZJ, Yan JJ, et al. Characterization and expression analysis of a FvRan1 gene from Flammulina velutipes[J]. Acta Edulis Fungi, 2018, 25(1): 13-19, 127. | |
[21] |
Tao YX, Chen RL, Yan JJ, et al. A hydrophobin gene, Hyd9, plays an important role in the formation of aerial hyphae and primordia in Flammulina filiformis[J]. Gene, 2019, 706: 84-90.
doi: 10.1016/j.gene.2019.04.067 URL |
[22] |
Yan JJ, Chekanova J, Liu YY, et al. Reactive oxygen species distribution involved in stipe gradient elongation in the mushroom Flammulina filiformis[J]. Cells, 2022, 11(12): 1896.
doi: 10.3390/cells11121896 URL |
[23] |
Liu YY, Ma XB, Long Y, et al. Effects of β-1, 6-glucan synthase gene(FfGS6)overexpression on stress response and fruit body development in Flammulina filiformis[J]. Genes, 2022, 13(10): 1753.
doi: 10.3390/genes13101753 URL |
[24] |
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT Method[J]. Methods, 2001, 25(4): 402-408.
doi: 10.1006/meth.2001.1262 pmid: 11846609 |
[25] |
Floudas D, Binder M, Riley R, et al. The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes[J]. Science, 2012, 336(6089): 1715-1719.
doi: 10.1126/science.1221748 pmid: 22745431 |
[26] | 朱刚, 吴林, 陈明杰, 等. 草菇锰过氧化物酶编码基因生物信息学分析及其转录水平和酶活性的测定[J]. 菌物学报, 2013, 32(5): 919-927. |
Zhu G, Wu L, Chen MJ, et al. Bioinformatic, gene expression and enzyme activity analyses of manganese peroxidases in Volvariella volvacea[J]. Mycosystema, 2013, 32(5): 919-927. | |
[27] | 江明锋, 张义正. 培养于天然冷杉木片的黄孢原毛平革菌木质素过氧化物酶基因表达的RT-PCR分析[J]. 微生物学报, 2003, 43(1): 65-72. |
Jiang MF, Zhang YZ. RT-PCR analysis of Phanerochaete chrysosporium lip genes in colonized fir wood[J]. Acta Microbiol Sin, 2003, 43(1): 65-72. | |
[28] |
Li H, Poulos TL. Structural variation in heme enzymes: a comparative analysis of peroxidase and P450 crystal structures[J]. Structure, 1994, 2(6): 461-464.
pmid: 7922023 |
[29] | Nakazawa T, Yamaguchi I, Zhang YF, et al. Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9[J]. Environ Microbiol, 2023, 1-16. |
[30] |
Martı́nez AT. Molecular biology and structure-function of lignin-degrading heme peroxidases[J]. Enzyme Microb Technol, 2002, 30(4): 425-444.
doi: 10.1016/S0141-0229(01)00521-X URL |
[31] |
Ruiz-Dueñas FJ, Camarero S, Pérez-Boada M, et al. A new versatile peroxidase from Pleurotus[J]. Biochem Soc Trans, 2001, 29(Pt 2): 116-122.
doi: 10.1042/bst0290116 URL |
[32] |
Takemoto D, Kamakura S, Saikia S, et al. Polarity proteins Bem1 and Cdc24 are components of the filamentous fungal NADPH oxidase complex[J]. Proc Natl Acad Sci USA, 2011, 108(7): 2861-2866.
doi: 10.1073/pnas.1017309108 pmid: 21282602 |
[33] |
严俊杰, 仝宗军, 刘媛媛, 等. 草菇芳香醇氧化酶基因vvaao1的序列特征与差异表达[J]. 生物技术通报, 2018, 34(4): 107-114.
doi: 10.13560/j.cnki.biotech.bull.1985.2018-0166 |
Yan JJ, Tong ZJ, Liu YY, et al. Sequence characterization and differential expression analysis of a aryl alcohol oxidase gene vvaao1 from Volvariella volvacea[J]. Biotechnol Bull, 2018, 34(4): 107-114. | |
[34] |
Mu DS, Li CY, Zhang XC, et al. Functions of the nicotinamide adenine dinucleotide phosphate oxidase family in Ganoderma lucidum: an essential role in ganoderic acid biosynthesis regulation, hyphal branching, fruiting body development, and oxidative-stress resistance[J]. Environ Microbiol, 2014, 16(6): 1709-1728.
doi: 10.1111/emi.2014.16.issue-6 URL |
[35] |
Hernández-Oñate MA, Esquivel-Naranjo EU, Mendoza-Mendoza A, et al. An injury-response mechanism conserved across Kingdoms determines entry of the fungus Trichoderma atroviride into development[J]. Proc Natl Acad Sci USA, 2012, 109(37): 14918-14923.
doi: 10.1073/pnas.1209396109 pmid: 22927395 |
[36] |
Yan JJ, Tong ZJ, Liu YY, et al. The NADPH oxidase in Volvariella volvacea and its differential expression in response to mycelial ageing and mechanical injury[J]. Braz J Microbiol, 2020, 51(1): 87-94.
doi: 10.1007/s42770-019-00165-4 |
[37] | 郑永德. 金针菇与杏鲍菇工厂化栽培工艺路线比较[J]. 中国食用菌, 2021, 40(6): 41-49, 55. |
Zheng YD. Comparison of factory cultivation technology between Flammulina velutipes and Pleurotus eryngii[J]. Edible Fungi China, 2021, 40(6): 41-49, 55. | |
[38] | 边银丙. 食用菌工厂化生产的关键技术及其研发方向[J]. 食药用菌, 2013, 21(3): 139-143. |
Bian YB. Key technologies and research and development direction of industrial production of edible fungi[J]. Edible Med Mushrooms, 2013, 21(3): 139-143. | |
[39] | 崔世瑞. 皂苷与斑玉蕈抗氧化酶活性相关性及其转录水平上的初步分析[D]. 南京: 南京农业大学, 2015. |
Cui SR. Correlation between saponin and antioxidant enzyme activity and preliminary analysis in transcriptional level[D]. Nanjing: Nanjing Agricultural University, 2015. | |
[40] | 任艳芳, 何俊瑜, 杨军, 等. 外源H2O2对盐胁迫下小白菜种子萌发和幼苗生理特性的影响[J]. 生态学报, 2019, 39(20): 7745-7756. |
Ren YF, He JY, Yang J, et al. Effects of exogenous hydrogen peroxide on seed germination and physiological characteristics of pakchoi seedlings(Brassica chinensis L.) under salt stress[J]. Acta Ecol Sin, 2019, 39(20): 7745-7756. |
[1] | SUN Ming-hui, WU Qiong, LIU Dan-dan, JIAO Xiao-yu, WANG Wen-jie. Cloning and Expression Analysis of CsTMFs Gene in Tea Plant [J]. Biotechnology Bulletin, 2023, 39(7): 151-159. |
[2] | ZHAO Xue-ting, GAO Li-yan, WANG Jun-gang, SHEN Qing-qing, ZHANG Shu-zhen, LI Fu-sheng. Cloning and Expression of AP2/ERF Transcription Factor Gene ShERF3 in Sugarcane and Subcellular Localization of Its Encoded Protein [J]. Biotechnology Bulletin, 2023, 39(6): 208-216. |
[3] | LIU Kui, LI Xing-fen, YANG Pei-xin, ZHONG Zhao-chen, CAO Yi-bo, ZHANG Ling-yun. Functional Study and Validation of Transcriptional Coactivator PwMBF1c in Picea wilsonii [J]. Biotechnology Bulletin, 2023, 39(5): 205-216. |
[4] | JIANG Qing-chun, DU Jie, WANG Jia-cheng, YU Zhi-he, WANG Yun, LIU Zhong-yu. Expression and Function Analysis of Transcription Factor PcMYB2 from Polygonum cuspidatum [J]. Biotechnology Bulletin, 2023, 39(5): 217-223. |
[5] | YAO Zi-ting, CAO Xue-ying, XIAO Xue, LI Rui-fang, WEI Xiao-mei, ZOU Cheng-wu, ZHU Gui-ning. Screening of Reference Genes for RT-qPCR in Neoscytalidium dimidiatum [J]. Biotechnology Bulletin, 2023, 39(5): 92-102. |
[6] | WANG Yi-qing, WANG Tao, WEI Chao-ling, DAI Hao-min, CAO Shi-xian, SUN Wei-jiang, ZENG Wen. Identification and Interaction Analysis of SMAS Gene Family in Tea Plant(Camellia sinensis) [J]. Biotechnology Bulletin, 2023, 39(4): 246-258. |
[7] | WEI Ming WANG Xin-yu WU Guo-qiang ZHAO Meng. The Role of NAD-dependent Deacetylase SRT in Plant Epigenetic Inheritance Regulation [J]. Biotechnology Bulletin, 2023, 39(4): 59-70. |
[8] | LIU Si-jia, WANG Hao-nan, FU Yu-chen, YAN Wen-xin, HU Zeng-hui, LENG Ping-sheng. Cloning and Functional Analysis of LiCMK Gene in Lilium ‘Siberia’ [J]. Biotechnology Bulletin, 2023, 39(3): 196-205. |
[9] | WANG Tao, QI Si-yu, WEI Chao-ling, WANG Yi-qing, DAI Hao-min, ZHOU Zhe, CAO Shi-xian, ZENG Wen, SUN Wei-jiang. Expression Analysis and Interaction Protein Validation of CsPPR and CsCPN60-like in Albino Tea Plant(Camellia sinensis) [J]. Biotechnology Bulletin, 2023, 39(3): 218-231. |
[10] | PANG Qiang-qiang, SUN Xiao-dong, ZHOU Man, CAI Xing-lai, ZHANG Wen, WANG Ya-qiang. Cloning of BrHsfA3 in Chinese Flowering Cabbage and Its Responses to Heat Stress [J]. Biotechnology Bulletin, 2023, 39(2): 107-115. |
[11] | MIAO Shu-nan, GAO Yu, LI Xin-ru, CAI Gui-ping, ZHANG Fei, XUE Jin-ai, JI Chun-li, LI Run-zhi. Functional Analysis of Soybean GmPDAT1 Genes in the Oil Biosynthesis and Response to Abiotic Stresses [J]. Biotechnology Bulletin, 2023, 39(2): 96-106. |
[12] | YAN Xiong-ying, WANG Zhen, WANG Xia, YANG Shi-hui. Microbial Sulfur Metabolism and Stress Resistance [J]. Biotechnology Bulletin, 2023, 39(11): 150-167. |
[13] | ZHANG Hong-hong, FANG Xiao-feng. Advances in the Regulation of Stress Sensing and Responses by Phase Separation in Plants [J]. Biotechnology Bulletin, 2023, 39(11): 44-53. |
[14] | GE Wen-dong, WANG Teng-hui, MA Tian-yi, FAN Zhen-yu, WANG Yu-shu. Genome-wide Identification of the PRX Gene Family in Cabbage(Brassica oleracea L. var. capitata)and Expression Analysis Under Abiotic Stress [J]. Biotechnology Bulletin, 2023, 39(11): 252-260. |
[15] | YANG Xu-yan, ZHAO Shuang, MA Tian-yi, BAI Yu, WANG Yu-shu. Cloning of Three Cabbage WRKY Genes and Their Expressions in Response to Abiotic Stress [J]. Biotechnology Bulletin, 2023, 39(11): 261-269. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||